Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 7708725Journal NLM ID: 7505876Publisher: National Academy of Sciences
Institutions: Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
Several mutants of Schizosaccharomyces pombe were obtained that are defective in protein glycosylation. One of the mutants, strain Sp550, makes galactomannoproteins with about half of the wild-type amount of galactose, whereas another strain, Sp137, makes glycoproteins that are almost devoid of galactose. Nondenaturing gel electrophoresis of cell extracts of both mutants revealed that they make invertases with a greatly increased mobility relative to the wild type. Additional study showed that Sp137 invertase has a subunit molecular mass that is about half that reported for the wild-type enzyme, owing to a reduction in carbohydrate content, whereas the native multimeric state appears unaltered. Structural studies on bulk cell-wall glycoprotein from Sp137 showed that the N-linked carbohydrate chains consist of a typical branched core oligosaccharide to which is attached an unsubstituted α 1-->6-polymannose outer chain. Consequently, the cells are agglutinated by antibodies against α 1-->6-linked mannose and have N-linked carbohydrate chains that are structurally analogous to the mnn2 mutant of Saccharomyces cerevisiae.
Structure type: homopolymer
Location inside paper: abstract
Trivial name: LAM, mannan, galactomannan, cell wall heteromannan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, N-glycan, cell surface polysaccharide, mannan, D-mannan, galactomannan
Contained glycoepitopes: IEDB_130701,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_76933,IEDB_983930,SB_198,SB_44,SB_67,SB_72
Methods: HPAEC, HPLC, enzymatic digestion
Biosynthesis and genetic data: genetic data
Related record ID(s): 147722, 147724, 147725
NCBI Taxonomy refs (TaxIDs): 4896Reference(s) to other database(s): GTC:G55317BB, GlycomeDB:
6776, CCSD:
46066, CBank-STR:6345
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error