Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 30448500Publication DOI: 10.1016/j.ijbiomac.2018.11.149Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Y.A. Knirel <yknirel
gmail.com>
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russian Federation, Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russian Federation, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russian Federation, S. N. Winogradsky Institute of Microbiology, Federal Research Centre 'Fundamentals of Biotechnology', Russian Academy of Sciences, Moscow, Russia, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Moscow, Russian Federation
Escherichia coli F17 isolated from horse feces was studied in respect to the O antigen (O polysaccharide) structure and genetics. The lipopolysaccharide was isolated by phenol-water extraction of bacterial cells and cleaved by mild acid hydrolysis to yield the O polysaccharide, which was studied by sugar analysis and selective solvolysis with CF3CO2H along with one- and two-dimensional 1H and 13C NMR spectroscopy. The O polysaccharide was found to have a branched pentasaccharide repeat (O-unit) containing one residue each of d-galactose, d-mannose, l-rhamnose, d-glucuronic acid, and N-acetyl-d-glucosamine; about 2/3?units bear a side-chain glucose residue. To our knowledge, the F17 O-polysaccharide structure established is unique among known bacterial polysaccharide structures. The O-antigen gene cluster of E. coli F17 between the conserved genes galF and gnd was sequenced and found to be 99% identical to that of E. coli 102,755 assigned to a novel OgN8 genotype (A. Iguchi, S. Iyoda, K. Seto, H. Nishii, M. Ohnishi, H. Mekata, Y. Ogura, T. Hayashi, Front. Microbiol. 7 (2016) 765). Genes in the cluster were annotated taking into account the F17 O-polysaccharide structure. The data obtained confirm that E. coli F17 and E. coli strains belonging to the OgN8 genotype can be considered as a candidate to a new E. coli O-serogroup. The O antigen of this novel type was demonstrated to make for an effective shield protecting the intimate outer membrane surface of bacteria from direct interaction with bacteriophages.
Lipopolysaccharide, Escherichia coli, O polysaccharide, bacterial polysaccharide structure, bacteriophage, O antigen gene cluster
Structure type: polymer chemical repeating unit
Location inside paper: p.391, fig.3, O39
The structure in this paper was incorrect:
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130701,IEDB_136105,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_144983,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983930,SB_44,SB_67,SB_7,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, ESI-MS, GPC, acid degradation, function analysis of gene clusters, trifluoroacetic acid solvolysis
Comments, role: structure from ref.[17], DOI:10.1016/j.carres.2014.02.013 in ID 30116.
Related record ID(s): 1054, 1478, 1479, 1480, 1481
NCBI Taxonomy refs (TaxIDs): 562
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There is only one chemically distinct structure:
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