Although there have been decades of research on streptococcus pneumoniae, it is still among the leading cause of infectious disease in the world. As a type of capsular polysaccharide (CPS) of streptococcus pneumoniae, pneumococcal polysaccharides are essential components for colonization and virulence in mammalian hosts. This study aimed to characterize the CPS structure of type 8 streptococcus pneumoniae, which is one of the most fatal serotypes. In this work, heparinase I&III was used to successfully digest pneumococcal type 8 polysaccharide (Pn8P). We characterized the oligosaccharide generated from the enzymatic depolymerization of Pn8P by size exclusion chromatography, mass spectrometry and nuclear magnetic resonance. This is the first study to enzymatically depolymerize and characterize Pn8P.
NMR, mass spectrometry, vaccine, pneumococcal polysaccharide, Heparinase, Size exclusion chromatography
NCBI PubMed ID: 32688016Publication DOI: 10.1016/j.carres.2020.108024Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: C. Ma
; W. Huang
Institutions: Department of Chemistry, Georgia State University, Atlanta, GA, 30303, United States, Analytical Research and Development, BioTherapeutics Pharmaceutical Sciences, Pfizer, Inc., Chesterfield, MO, USA, Georgia Highlands College, 5901 Stewart Pkwy, Douglasville, GA, 30135, United States
Methods: 13C NMR, 1H NMR, NMR-2D, ESI-MS, enzymatic degradation, SEC, HPLC-ELSD, HPLC-RI-MS
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