Lipopolysaccharide (LPS) of Ochrobactrum cytisi IPA7.2, a bacterium isolated from the roots of Solanum tuberosum L., was extracted from dry bacterial cells and chemically characterized. The O-specific polysaccharide was obtained by mild acid hydrolysis of the LPS and studied by sugar analysis and (1)H and (13)C NMR spectroscopy, including (1)H,(1)H COSY, (1)H,(1)H TOCSY, (1)H,(1)H ROESY, (1)H,(13)C HSQC, and (1)H,(13)C HMBC experiments. The polysaccharide was linear and consisted of trisaccharide repeating units of the following structure: A putative O-antigen gene cluster of O. cytisi IPA7.2 was identified and found to be consistent with the O-specific polysaccharide structure. The LPS of Ochrobactrum cytisi IPA7.2 promoted the growth of potato microplants in vitro.
Lipopolysaccharide, O-Polysaccharide structure, Ochrobactrum cytisi, O-antigen biosynthesis gene cluster, Potato microplants
NCBI PubMed ID: 31730968Publication DOI: 10.1016/j.ijbiomac.2019.11.017Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: si_elena@mail.ru
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russia, Vavilov Saratov State Agrarian University, Saratov 410012, Russia, Chernyshevsky Saratov State University, Saratov 410012, Russia
Methods: 13C NMR, 1H NMR, NMR-2D, SDS-PAGE, GLC, mild acid hydrolysis, GC, de-O-acetylation, composition analysis, GPC, function analysis of gene clusters
Found