The bacteria from Ochrobactrum genus are commonly found in the soil and association with the roots of plants. The O-polysaccharide and the glucan were isolated from the Ochrobactrum rhizosphaerae PR17(T) strain. Purified polysaccharides were analysed using chemical methods and NMR spectroscopy. Sugar and absolute configuration assignment combined with NMR data revealed the chemical structure of the repeating unit of the O- [formula: see text].
NMR, structure, O-polysaccharide, glucan, Ochrobactrum rhizosphaerae PR17(T)
NCBI PubMed ID: 32889436Publication DOI: 10.1016/j.carres.2020.108136Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: zbigniew.kaczynski@ug.edu.pl
Institutions: Faculty of Chemistry, University of Gdansk, Gdansk, Poland, Laboratory of Biological Plant Protection, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk, University of Gdansk, Gdansk, Poland
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, acid hydrolysis, GLC, composition analysis, acetylation, SEC, reduction with NaBH4