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Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)

The structure was elucidated in this paper
NCBI PubMed ID: 9345753
Journal NLM ID: 0043535
Publisher: Elsevier
Institutions: Centro de Investigaciones Biologicas, CSIC, Velazquez 144, 28006 Madrid, Spain, Grupo de carbohidratos, Institute de Quimica Organica, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain

The structure of acidic fungal polysaccharides, isolated from the cell wall of Cylindrocladium and Calonectria species, has been investigated by chemical analysis, methylation and reductive cleavage analyses, and ID and 2D H and C NMR spectroscopy. The polysaccharides have an idealized repeating unit: linked to a small mannan core (< 5%), with n = 3 for Cylindrocladium penicilloides and C. quinqueseptatum and n = 5 for Calonectria theae, C. crotalariae, and C. colhounii.

structural, polysaccharide, polysaccharides, acidic, cell wall, elucidation, galactofuranose, fungal, structurel

Structure type: polymer chemical repeating unit
Aglycon: small mannan core
Trivial name: fungal polysaccharide
Contained glycoepitopes: IEDB_115136,IEDB_136095,IEDB_137472,IEDB_140630,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_149176,IEDB_190606,IEDB_983931,SB_192

Methods: NMR-2D, methylation, GC-MS, Taylor-Conrad reduction

Related record ID(s): 198
NCBI Taxonomy refs (TaxIDs): 182850, 131354
Reference(s) to other database(s): GTC:G74747NW
Show glycosyltransferases

NMR conditions: in D2O at 313 K [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
6,6,6,2	aDGlcp	99.0	72.0	73.7	70.5	73.3	61.4
6,6,6	bDGalf	107.2	87.4	76.3	83.4	70.5	70.2
6,6,2	aDGlcp	99.0	72.0	73.7	70.5	73.3	61.4
6,6	bDGalf	107.2	87.4	76.3	83.4	70.5	70.2
6,2	aDGlcp	99.0	72.0	73.7	70.5	73.3	61.4
6	bDGalf	107.2	87.4	76.3	83.4	70.5	70.2
2	aDGlcpA	99.0	71.9	73.4	72.8	73.5	176.2
	bDGalf	107.2	87.4	76.3	83.4	70.5	70.2


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
6,6,6,2	aDGlcp	5.06	3.57	3.71	3.44	3.77	3.76-3.87
6,6,6	bDGalf	5.17	4.18	4.23	4.03	3.99	3.65-3.91
6,6,2	aDGlcp	5.06	3.57	3.71	3.44	3.77	3.76-3.87
6,6	bDGalf	5.17	4.18	4.23	4.03	3.99	3.65-3.91
6,2	aDGlcp	5.06	3.57	3.71	3.44	3.77	3.76-3.87
6	bDGalf	5.17	4.18	4.23	4.03	3.99	3.65-3.91
2	aDGlcpA	5.06	3.61	3.74	3.54	4.04	-
	bDGalf	5.17	4.18	4.23	4.03	3.99	3.65-3.91


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
6,6,6,2	aDGlcp	99.0/5.06	72.0/3.57	73.7/3.71	70.5/3.44	73.3/3.77	61.4/3.76-3.87
6,6,6	bDGalf	107.2/5.17	87.4/4.18	76.3/4.23	83.4/4.03	70.5/3.99	70.2/3.65-3.91
6,6,2	aDGlcp	99.0/5.06	72.0/3.57	73.7/3.71	70.5/3.44	73.3/3.77	61.4/3.76-3.87
6,6	bDGalf	107.2/5.17	87.4/4.18	76.3/4.23	83.4/4.03	70.5/3.99	70.2/3.65-3.91
6,2	aDGlcp	99.0/5.06	72.0/3.57	73.7/3.71	70.5/3.44	73.3/3.77	61.4/3.76-3.87
6	bDGalf	107.2/5.17	87.4/4.18	76.3/4.23	83.4/4.03	70.5/3.99	70.2/3.65-3.91
2	aDGlcpA	99.0/5.06	71.9/3.61	73.4/3.74	72.8/3.54	73.5/4.04	
	bDGalf	107.2/5.17	87.4/4.18	76.3/4.23	83.4/4.03	70.5/3.99	70.2/3.65-3.91

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
6,6,6,2	aDGlcp	5.06	3.57	3.71	3.44	3.77	3.76 3.87
6,6,6	bDGalf	5.17	4.18	4.23	4.03	3.99	3.65 3.91
6,6,2	aDGlcp	5.06	3.57	3.71	3.44	3.77	3.76 3.87
6,6	bDGalf	5.17	4.18	4.23	4.03	3.99	3.65 3.91
6,2	aDGlcp	5.06	3.57	3.71	3.44	3.77	3.76 3.87
6	bDGalf	5.17	4.18	4.23	4.03	3.99	3.65 3.91
2	aDGlcpA	5.06	3.61	3.74	3.54	4.04
	bDGalf	5.17	4.18	4.23	4.03	3.99	3.65 3.91

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
6,6,6,2	aDGlcp	99.0	72.0	73.7	70.5	73.3	61.4
6,6,6	bDGalf	107.2	87.4	76.3	83.4	70.5	70.2
6,6,2	aDGlcp	99.0	72.0	73.7	70.5	73.3	61.4
6,6	bDGalf	107.2	87.4	76.3	83.4	70.5	70.2
6,2	aDGlcp	99.0	72.0	73.7	70.5	73.3	61.4
6	bDGalf	107.2	87.4	76.3	83.4	70.5	70.2
2	aDGlcpA	99.0	71.9	73.4	72.8	73.5	176.2
	bDGalf	107.2	87.4	76.3	83.4	70.5	70.2

There is only one chemically distinct structure:

[*]OC[C@@H](O)[C@@H]1O[C@@H](OC[C@@H](O)[C@@H]2O[C@@H](OC[C@@H](O)[C@@H]3O[C@@H](OC[C@@H](O)[C@@H]4O[C@@H]([*])[C@H](O[C@H]5O[C@H](C(=O)O)[C@@H](O)[C@H](O)[C@H]5O)[C@H]4O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H]3O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H]2O)[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H]1O

SVG MW SMILES 3D mol Ionize

structural, polysaccharide, polysaccharides, acidic, cell wall, elucidation, galactofuranose, fungal, structurel

Structure type: polymer chemical repeating unit
Aglycon: small mannan core
Contained glycoepitopes: IEDB_115136,IEDB_136095,IEDB_137472,IEDB_140630,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_149176,IEDB_190606,IEDB_983931,SB_192

Methods: NMR-2D, methylation, GC-MS, Taylor-Conrad reduction

Related record ID(s): 15
NCBI Taxonomy refs (TaxIDs): 57138, 131350
Reference(s) to other database(s): GTC:G30735WX
Show glycosyltransferases

There is only one chemically distinct structure:

[*]OC[C@@H](O)[C@@H]1O[C@@H](OC[C@@H](O)[C@@H]2O[C@@H](OC[C@@H](O)[C@@H]3O[C@@H](OC[C@@H](O)[C@@H]4O[C@@H](OC[C@@H](O)[C@@H]5O[C@@H](OC[C@@H](O)[C@@H]6O[C@@H]([*])[C@H](O[C@H]7O[C@H](C(=O)O)[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)[C@H]5O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@H]4O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H]3O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H]2O)[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H]1O

SVG MW SMILES 3D mol Ionize