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Structure type: oligomer
Trivial name: common core of GPI anchors
Compound class: GPI-anchor
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141793,IEDB_141807,IEDB_141829,IEDB_143632,IEDB_144983,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_474450,IEDB_983930,SB_136,SB_191,SB_196,SB_198,SB_44,SB_67,SB_72

• Article ID: 5925
  Ferguson MAJ, Hart GW, Kinoshita T "Glycosylphosphatidylinositol Anchors" - Book: In: Essentials of Glycobiology [Internet] (2017)
  

  Plasma membrane proteins are either peripheral proteins or integral membrane proteins. The latter include proteins that span the lipid bilayer once or several times, and another class that are covalently attached to lipids. Proteins attached to glycosylphosphatidylinositol (GPI) via their carboxyl termini are generally found in the outer leaflet of the lipid bilayer and face the extracellular environment. The GPI membrane anchor may be conveniently thought of as an alternative to the single transmembrane domain of type-1 integral membrane proteins. This chapter reviews the discovery, distribution, structure, biosynthesis, properties, and suggested functions of GPI membrane anchors and related molecules.

  proteins, Glycosylphosphatidylinositol, glycobiology, GPI, membrane protein

  NCBI PubMed ID: 28876821
  Publication DOI: 10.1101/glycobiology.3e.012
  Publisher: Cold Spring Harbor (NY), Cold Spring Harbor Laboratory Press
  Correspondence: majferguson@dundee.ac.uk
  Editors: Varki A, Cummings RD, Esko JD, Stanley P, Hart GW, Aebi M, Darvill AG, Kinoshita T, Packer NH, Prestegard JH, Schnaar RL, Seeberger PH
  Institutions: Division of Biological Chemistry and Drug Discovery, Wellcome Trust Biocentre, College of Life Science, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom, Johns Hopkins University, Baltimore, Maryland, USA, Laboratory of Immunoglycobiology, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
  
   
  
  (Euglenozoa)
  CSDB ID 7295 (all data & tools)

Show legend Show as text

Structure type: oligomer
Trivial name: variant surface glycoprotein (VSG) GPI-anchor
Compound class: GPI-anchor
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141181,IEDB_141793,IEDB_141807,IEDB_141829,IEDB_143632,IEDB_144983,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_474450,IEDB_983930,SB_136,SB_191,SB_196,SB_198,SB_44,SB_67,SB_72

• Article ID: 6010
  Swarts BM, Guo ZW "Chemical synthesis and functionalization of clickable glycosylphosphatidylinositol anchors" - Chemical Science 2(12) (2011) 2342-2352
  

  Glycosylphosphatidylinositol (GPI) anchorage is a common posttranslational modification of eukaryotic proteins. Chemical synthesis of structurally defined GPIs and GPI derivatives is a necessary step toward understanding the properties and functions of these molecules in biological systems. In this work, the synthesis of several functionalized GPI anchors was accomplished using the paramethoxybenzyl (PMB) group for permanent hydroxyl protection, which allowed the incorporation of functionalities that are incompatible with permanent protecting groups traditionally used in carbohydrate synthesis. A flexible convergent-divergent assembly strategy enabled efficient access to a diverse set of target structures, including 'clickable'' alkyne- and azide-modified GPIs. For global deprotection, a one-pot reaction was employed to afford the target GPIs in excellent yields (85-97%). Fully deprotected clickable GPI derivatives were readily conjugated to imaging and affinity probes via Cu(I)-catalyzed and Cu-free strain-promoted [3 + 2] cycloaddition to result in GPI-Flour and GPI-Biotin conjugates, respectively.

  synthesis, carbohydrate, proteins, Glycosylphosphatidylinositol, GPI, GPI anchor

  Publication DOI: 10.1039/C1SC00440a
  Journal NLM ID: 101545951
  Publisher: Cambridge, UK: Royal Society of Chemistry
  Correspondence: zwguo@chem.wayne.edu
  Institutions: Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, Michigan, USA
  Methods: 13C NMR, 1H NMR, TLC, 31P NMR, chemical synthesis, MALDI-TOF MS, UV, optical rotation measurement, SEC, CC, conjugation
  
   
  
  (Euglenozoa)
  CSDB ID 7380 (all data & tools)