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1. (Article ID: 1526)
 
Katzenellenbogen E, Kocharova NA, Zatonsky GV, Shashkov AS, Knirel YA, Gamian A, Bogulska M
Structure of the O-polysaccharide of Hafnia alvei strain PCM 1189 that has hexa- to octa-saccharide repeating units owing to incomplete glucosylation
Carbohydrate Research 340(2) (2005) 263-270
 

The O-polysaccharide of Hafnia alvei PCM 1189 consists of D-glucose, D-galactose, D-GalNAc and D-GlcA and lacks the strict regularity. The intact and carboxyl-reduced polysaccharides as well as oligosaccharides obtained by partial acid hydrolysis were studied by chemical and enzymatic analyses, methylation and NMR spectroscopy. The following structure was established for the O-polysaccharide, which is built up of branched hexa- to octasaccharide repeating units differing in the number of lateral glucose residues: [structure: see text] where the glucose residues shown in italics are nonstoichiometric substituents. The repeating units include also a minor O-acetyl group, whose position was not determined.

Lipopolysaccharide, O-antigen, Hafnia alvei, enterobacteria, bacterial polysaccharide structure

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