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1. (Article ID: 1549)
Ortega X, Hunt TA, Loutet S, Vinion-Dubiel AD, Datta A, Choudhury B, Goldberg JB, Carlson R, Valvano MA
Reconstitution of O-Specific Lipopolysaccharide Expression in Burkholderia cenocepacia Strain J2315, Which Is Associated with Transmissible Infections in Patients with Cystic Fibrosis
Journal of Bacteriology 187(4) (2005)
1324-1333
Burkholderia cenocepacia is an opportunistic bacterium that infects patients with cystic fibrosis. B. cenocepacia strains J2315, K56-2, C5424, and BC7 belong to the ET12 epidemic clone, which is transmissible among patients. We have previously shown that transposon mutants with insertions within the O antigen cluster of strain K56-2 are attenuated for survival in a rat model of lung infection. From the genomic DNA sequence of the O antigen-deficient strain J2315, we have identified an O antigen lipopolysaccharide (LPS) biosynthesis gene cluster that has an IS402 interrupting a predicted glycosyltransferase gene. A comparison with the other clonal isolates revealed that only strain K56-2, which produced O antigen and displayed serum resistance, lacked the insertion element inserted within the putative glycosyltransferase gene. We cloned the uninterrupted gene and additional flanking sequences from K56-2 and conjugated this plasmid into strains J2315, C5424, and BC7. All the exconjugants recovered the ability to form LPS O antigen. We also determined that the structure of the strain K56-2 O antigen repeat, which was absent from the LPS of strain J2315, consisted of a trisaccharide unit made of rhamnose and two N-acetylgalactosamine residues. The complexity of the gene organization of the K56-2 O antigen cluster was also investigated by reverse transcription-PCR, revealing several transcriptional units, one of which also contains genes involved in lipid A-core oligosaccharide biosynthesis
Burkholderia, O-antigen, gene cluster, glycosyltransferase, clone, cystic fibrosis, lipopolysaccharide expression
NCBI PubMed ID: 15687196Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: mvalvano
uwo.ca
Institutions: Department of Microbiology and Immunology, University of Western Ontario, Dental Sciences Building Room 3014, London, Ontario, Canada N6A 5C1, Department of Microbiology, University of Virginia Health System, Charlottesville, Virginia2, Department of Biochemistry and Molecular Biology3 and Complex Carbohydrate Research Center,4 University of Georgia, Athens, Georgia
Methods: NMR, composition analysis, genetic methods
The publication contains the following compound(s):
- Compound ID: 4181
Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Reference(s) to other database(s): GTC:G06700QW, GlycomeDB:
27636
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