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1. (Article ID: 1565)
 
St-Michael F, Li J, Howard MD, Duncan AJ, Inzana TJ, Cox AD
Structural analysis of the oligosaccharide of Histophilus somni (Haemophilus somnus) strain 2336 and identification of several lipooligosaccharide biosynthesis gene homologues
Carbohydrate Research 340(4) (2005) 665-672
 

The structure of the core oligosaccharide from a pneumonic Histophilus somni (Haemophilus somnus) strain 2336 was elucidated. The lipooligosaccharide (LOS) was subjected to a variety of degradative procedures. The structures of the purified products were established by monosaccharide and methylation analyses, NMR spectroscopy and mass spectrometry. The following structure for the core oligosaccharide was determined on the basis of the combined data from these experiments: The structural elucidation was intriguing as it suggested several differences in the LOS structures between strain 2336 and the related strain 738. Strain 738 originated following passaging of strain 2336 through a calf. The differences between the two structures are a different linkage between Gal II and GlcNAc (1→4 here; 1→3 in 738), the absence of phosphocholine (PCho) from 2336 and the presence of two phosphoethanolamine (PEtn) residues and Gal III (at the 2-position) of Hep II in 2336. Although pulse-field gel electrophoresis data following digest with only one restriction enzyme showed identical profiles suggesting that strains 738 and 2336 are the same strain, the structural data does suggest that, if strain 738 is indeed a phase variant of strain 2336, considerable variation occurred on calf passaging and could therefore be an intriguing example of how broadly this bacterium can adapt itself in the host

NMR, biosynthesis, Haemophilus, oligosaccharide, structure, core, Lipooligosaccharide, gene, host, LOS, phase, strain, structural, variation, analysis, Research, core oligosaccharide, NMR spectroscopy, bacteria, spectrometry, biological, linkage, structural analysis, identification, mass spectrometry, methylation, spectroscopy, Phosphocholine, elucidation, phosphoethanolamine, enzyme, difference, purified, variant, monosaccharide, gel electrophoresis, Haemophilus somnus, electrophoresis, gel, absence, profile, restriction

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