In this study Bacillus amyloliquefaciens RK3 was isolated from a sugar mill effluent-contaminated soil and utilised to generate a potential polysaccharide with anti-Alzheimer's activity. Traditional and molecular methods were used to validate the strain. The polysaccharide produced by B. amyloliquefaciens RK3 was purified, and the yield was estimated to be 10.35 gL-1. Following purification, the polysaccharide was structurally and chemically analysed. The structural analysis revealed the polysaccharide consists of α-d-mannopyranose (α-d-Manp) and β-d-galactopyranose (β-d-Galp) monosaccharide units connected through glycosidic linkages (i.e., β-d-Galp(1→6)β-d-Galp (1→6)β-d-Galp(1→2)β-d-Galp(1→2)[β-d-Galp(1→6)]β-d-Galp(1→2)α-d-Manp(1→6)α-d-Manp (1→6)α-d-Manp(1→6)α-d-Manp(1→6)α-d-Manp). The scanning electron microscopy and energy-dispersive X-ray spectroscopy imaging of polysaccharides emphasise their compactness and branching in the usual tubular heteropolysaccharide structure. The purified exopolysaccharide significantly impacted the plaques formed by the amyloid proteins during Alzheimer's disease. Further, the results also highlighted the potential applicability of exopolysaccharide in various industrial and pharmaceutical applications.
exopolysaccharide, Bacillus, biopolymer, Alzheimer's disease, biomedical application, cognitive function
NCBI PubMed ID: 34502882Publication DOI: 10.3390/polym13172842Journal NLM ID: 101545357Publisher: Basel: MDPI
Correspondence: sivasankar.ps@gmail.com; rkthampu@kakatiya.ac.in
Institutions: Department of Microbiology, Kakatiya University, Warangal 506009, India, Department of Zoology, School of Life Sciences, Periyar University, Salem 636011, India, Department of Environmental Science, School of Life Sciences, Periyar University, Salem 636011, India, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi Arabia, Medicinal Chemistry Division, University College of Pharmaceutical Sciences, Kakatiya University, Warangal 506009, India, Department of Life and Environmental Sciences, University of Cagliari, Monserrato, 09042 Cagliari, Italy, Department of Mechanical, Chemical and Material Engineering, University of Cagliari, Via Marengo 2, 09123 Cagliari, Italy, Department of Electrical and Electronic Engineering, University of Cagliari, Via Marengo 2, 09123 Cagliari, Italy, Institute for Plant Cell Biology and Biotechnology, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany
Methods: 13C NMR, 1H NMR, GC-MS, X-ray, sugar analysis, DNA techniques, TLC, FTIR, UV, extraction, SEM, physiological tests, anti-Alzheimer study
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