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1. (Article ID: 1580)
 
Vinogradov EV, Brade L, Brade H, Holst O
The structure of the O-specific polysaccharide of the lipopolysaccharide from Acinetobacter strain 44 (DNA group 3)
Polish Journal of Chemistry 79 (2005) 267-273
 

Extraction of dry bacteria of Acinetobacter strain 44 (DNA group 3) by phenol/water gave a polymer that was identified by means of serological studies as S-form lipopolysaccharide (LPS). Mild acetic acid hydrolysis degraded the O-specific polysaccharide, thus, its structure was investigated by compositional analyses and NMR spectroscopy after de-O-acylation and Smith degradation of the LPS. The structure of the O-specific polysaccharides was: [see formula in the text]. After immunization of BALB/c mice with Acinetobacter strain 44, monoclonal antibody S48-26 (IgG3 isotype) was obtained which reacted in Western blot with this LPSZ and characterized it as S-form.

Lipopolysaccharide, structure, DNA, strain, polysaccharide, repeating unit, group, Acinetobacter, O-polysaccharide, O polysaccharide, O-specific, O-specific polysaccharide, tyvelose, Enterobacter

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