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Structure type: polymer chemical repeating unit
Compound class: CPS
Contained glycoepitopes: IEDB_135813,IEDB_136105,IEDB_137340,IEDB_137485,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_152206,IEDB_225177,IEDB_885823,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_72

• Article ID: 6150
  Timoshina OY, Shneider MM, Evseev PV, Shchurova AS, Shelenkov AA, Mikhailova YV, Sokolova OS, Kasimova AA, Arbatsky NP, Dmitrenok AS, Knirel YA, Miroshnikov KA, Popova AV "Novel Acinetobacter baumannii Bacteriophage Aristophanes Encoding Structural Polysaccharide Deacetylase" - Viruses 13(9) (2021) 1688
  

  Acinetobacter baumannii appears to be one of the most crucial nosocomial pathogens. A possible component of antimicrobial therapy for infections caused by extremely drug-resistant A. baumannii strains may be specific lytic bacteriophages or phage-derived enzymes. In the present study, we observe the biological features, genomic organization, and phage-host interaction strategy of novel virulent bacteriophage Aristophanes isolated on A. baumannii strain having K26 capsular polysaccharide structure. According to phylogenetic analysis phage Aristophanes can be classified as a representative of a new distinct genus of the subfamily Beijerinckvirinae of the family Autographiviridae. This is the first reported A. baumannii phage carrying tailspike deacetylase, which caused O-acetylation of one of the K26 sugar residues.

  Acinetobacter baumannii, capsular polysaccharide, bacteriophage, deacetylase, capsular type

  NCBI PubMed ID: 34578271
  Publication DOI: 10.3390/v13091688
  Journal NLM ID: 101509722
  Publisher: Basel, Switzerland: MDPI
  Correspondence: popova_nastya86@mail.ru
  Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia, Central Scientific Research Institute of Epidemiology, Novogireevskaya 3a, 111123 Moscow, Russia, Biology Department, Lomonosov Moscow State University, Leninskie Gory 1, 119234 Moscow, Russia, Moscow Institute of Physics and Technology, National Research University, Institutskiy per. 9, Dolgoprudny, 141700 Moscow, Russia, Biology Department, Shenzhen MSU-BIT University, 1 International University Park Road, Longgang District, Shenzhen 518172, China, State Research Center for Applied Microbiology and Biotechnology, 24 'Quarter A' Territory, Obolensk, City District Serpukhov, 142279 Moscow, Russia
  Methods: 13C NMR, 1H NMR, NMR-2D, PCR, de-O-acetylation, GPC, bioinformatic analysis, phylogenetic analysis, sequencing, TEM, phage characterization, depolymerization by phage, phage genome annotation
  
   
  
  Acinetobacter baumannii K26 KZ-1098
  CSDB ID 10951 (all data & tools)
• Article ID: 6309
  Rudenko N, Karatovskaya A, Zamyatina A, Shepelyakovskaya A, Semushina S, Brovko F, Shpirt A, Torgov V, Kolotyrkina N, Zinin A, Kasimova A, Perepelov A, Shneider M, Knirel Y "Immune Response to Conjugates of Fragments of the Type K9 Capsular Polysaccharide of Acinetobacter baumannii with Carrier Proteins" - Microbiology Spectrum 10(5) (2022) e0167422
  

  The clonal bacterial species Acinetobacter baumannii is an emerging multidrug-resistant pathogen which causes high-lethality infections. Cells of A. baumannii are surrounded by the type-specific capsular polysaccharide (CPS), which provides resistance to the protective mechanisms of the host and is considered a target for immunization. The conjugates of three inert carrier proteins and A. baumannii type K9 CPS fragments, which contained various numbers of oligosaccharide repeats (K-units), were synthesized by periodate oxidation and squaric acid chemistry. The conjugates were applied to immunize mice, and chemical synthesis by squaric acid was shown to significantly improve the immunogenic properties of glycoconjugate. In BALB/c mice, IgG antibodies were predominant among type K9 CPS reactive antibodies, and their total content was several times higher than that of IgM. Immune sera were characterized by their opsonization ability during practically the entire lives of the experimental mice. The sera were cross-reactive, but the highest specificity was observed against the antigen (type K9 CPS) used for immunization. The immunization of BALB/c and ICR-1 mice with a glycoconjugate without adjuvants led to varying degrees of stimulation of IL-10, IL-17A, and TNF-alpha production, but not IL-4 production in the ICR-1 mice. This is in contrast to the BALB/c mice, in which gamma-IFN production was also activated. The protective effectiveness of the glycoconjugates obtained by squaric acid chemistry was demonstrated by experiments that involved challenging immunized and nonimmunized animals with a lethal dose of A. baumannii K9. IMPORTANCE Immunization by glycoconjugates with A. baumannii type K9 CPS fragments induced a high level of antibodies (predominantly IgG) in sera, which reacted specifically with the CPS of A. baumannii type K9, as well as a long immunological memory. The sera of immunized animals efficiently opsonized A. baumannii type K9. Immunization resulted in the balanced production of pro/anti-inflammatory lymphokines and protective antibodies to ensure the survival of the mice infected with A. baumannii. The level of specific antibodies was sufficient to provide protective immunity against the challenge by A. baumannii, making this approach applicable in the development of vaccine preparations.

  carbohydrates, carbohydrate, Acinetobacter baumannii, capsular polysaccharide, glycoconjugate, immunochemistry, interleukins, opsonisation assay

  NCBI PubMed ID: 35980044
  Publication DOI: 10.1128/spectrum.01674-22
  Journal NLM ID: 101634614
  Publisher: Washington, DC: ASM Press
  Correspondence: N.Rudenko
  Institutions: Laboratory of Immunochemistry, Pushchino Branch, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow Region, Russia, Laboratory of Carbohydrates and Biocides, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Laboratory of Molecular Bioengineering, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
  Methods: periodate oxidation, EIA, chemical synthesis, MALDI-TOF MS, statistical analysis, immunization, HR-ESI-MS, opsonization assay
  
   
  
  Acinetobacter baumannii K128 KZ-1098
  CSDB ID 9191 (all data & tools)