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1. (Article ID: 1595)
Kawasaki K, Ernst RK, Miller SI
Purification and characterization of deacylated and/or palmitoylated lipid A species unique to Salmonella enterica serovar Typhimurium
Journal of Endotoxin Research 11(1) (2005)
57-61
The Salmonella enterica serovar Typhimurium virulence gene products PhoP/PhoQ sense host microenvironments to regulate the expression of a lipid A 3-O-deacylase, PagL, and a lipid A palmitoyltransferase, PagP. Therefore, deacylation and/or palmitoylation of lipid A could occur in Salmonellae adapted to host environments. The PhoP/PhoQ-regulated modification of lipid A alters host recognition and signaling, and may play an important role in host defense against Salmonellae infection. Here we report the purification and characterization of modified lipid A species. Deacylated lipid A, deacylated and palmitoylated lipid A, and palmitoylated lipid A species were generated in Escherichia coli cells heterologously expressing salmonellae PagL and/or PagP, and then purified by sequential thin-layer chromatography. The purified lipid A species showed m/z values that correspond to single lipid A species on mass spectrometry analysis. The modified lipid A species showed reduced ability to induce cellular signaling through Toll-like receptor 4, suggesting a specific function of the lipid A modifications in the pathogenesis of salmonellae infection.
deacylated lipid A, palmitoylated lipid A, Salmonella enterica serovar Typhimurium
NCBI PubMed ID: 15826380Publication DOI: 10.1179/096805105225006696Journal NLM ID: 9433350Publisher: Maney Publishing
Correspondence: kawa
nih.go.jp
Institutions: Departments of Microbiology, Medicine, and Genome Sciences, University of Washington, Seattle, Washington, USA
Methods: PCR, de-O-acylation, chemical methods, MALDI-TOF MS, biological assays, genetic methods, enzyme assay
The publication contains the following compound(s):
- Compound ID: 314
|
R-3HOMyr-(1-2)-+
|
Myr-(1-3)-R-3HOMyr-(1-3)-+ |
| |
Lau-(1-3)-R-3HOMyr-(1-2)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
| |
P-4)-+ |
|
R-3HOMyr-(1-3)-+ |
Show graphically |
Structure type: oligomer
Compound class: LPS, lipid A, glycolipid, phosphoglycolipid
- Compound ID: 1795
|
Pam-(1-3)-R-3HOMyr-(1-2)-+
|
Myr-(1-3)-R-3HOMyr-(1-3)-+ |
| |
Lau-(1-3)-R-3HOMyr-(1-2)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
| |
P-4)-+ |
|
R-3HOMyr-(1-3)-+ |
Show graphically |
Structure type: oligomer
Compound class: lipid A
- Compound ID: 1796
|
Pam-(1-3)-R-3HOMyr-(1-2)-+
|
Myr-(1-3)-R-3HOMyr-(1-3)-+ |
| |
Lau-(1-3)-R-3HOMyr-(1-2)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
|
P-4)-+ |
Show graphically |
Structure type: oligomer
Compound class: lipid A
- Compound ID: 1797
|
R-3HOMyr-(1-2)-+
|
Myr-(1-3)-R-3HOMyr-(1-3)-+ |
| |
Lau-(1-3)-R-3HOMyr-(1-2)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
|
P-4)-+ |
Show graphically |
Structure type: oligomer
Compound class: lipid A
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