A novel exopolysaccharide was isolated from Bacillus velezensis RSDM1, purified and characterized. The optimization was carried out by Box-Behnken design and the highest yield of EPS-RSDM1 was 1969.53 mg/L. The EPS was composed by glucose with a molecular weight of 1.78 x 104 Da and connected by alpha-1,6 and alpha-1,3. The EPS was thermally stable and showed pseudoplastic rheology. The SEM have demonstrated a porous structure. Furthermore, EPS-RSDM1 showed a protective effect against the oxidative stress induced by H2O2 in Tetrahymena thermophila. The exposure of the cells to H2O2 reduced significantly the cell survival and CAT, SOD, GR, SDH and GAPDH activities, and increased the MDA production. However, pretreatment of the cells with EPS-RSDM1 prior to H2O2 exposure elevated the cell survival and enzyme activities, and decreased the level of MDA activity. Our study suggest that EPS-RSDM1 could be useful for preventing cellular oxidation in pharmaceutical and food industries.
structure characterization, oxidative stress, Bacillus velezensis
NCBI PubMed ID: 36243160Publication DOI: 10.1016/j.ijbiomac.2022.10.095Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: B.El. Khalfi
Institutions: Laboratory of Physiopathology, Molecular Genetics & Biotechnology, Faculty of Sciences Ain Chock, Health and Biotechnology Research Centre, Hassan II University of Casablanca, Maarif B.P 5366, Casablanca, Morocco, Department of Food Science, University of Agricultural Science and Veterinary Medicine, 3-5 Calea Mana?tur, 400372 Cluj-Napoca, Romania, Molecular Nutrition and Proteomics Lab, CDS3, Life Science Institute, University of Agricultural Science and Veterinary Medicine, Calea Mana?tur 3-5, 400372, Cluj-Napoca, Romania, Food Biotechnology and Molecular Gastronomy, CDS7, Life Science Institute, University of Agricultural Science and Veterinary Medicine, Calea Mana?tur 3-5, 400372 Cluj-Napoca, Romania, Department of Chemistry, Laboratory of Organic Synthesis, Extraction, and Valorization, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Maarif B.P 5366, Casablanca, Morocco
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, X-ray, SDS-PAGE, Western blotting, FTIR, statistical analysis, enzymatic assay, cytotoxicity assay, SEM, DEPT, TGA, DTG, SEM-EDX
Found