Extracellular polysaccharide (EPS) produced by a deep-sea, psychrotolerant Bacillus altitudinis SORB11 was evaluated by considering physiochemical nature and structural constituents. The productivity of crude EPS was measured ~ 13.17 g L-1. The surface topography of the crude EPS showed a porous, webbed structure along with a branched coil-like configuration. The crystalline crude EPS contained a high amount of sulfur. Further, the crude EPS was subjected for purification. The molecular weight of purified EPS was determined ~ 9.8 × 104 Da. The purified EPS was appeared to show glucomannan-like configuration that is composed of →4)-β-Manp-(1→ and →4)-β-Glcp-(1→ residues. So, this polysaccharide was comparable to the structure of plant-derived glucomannan. Subsequently, EPS biosynthesis protein clusters like EpsC, EpsD, EpsE, and glycosyltransferase family proteins were predicted from the genome of strain SORB11, which may provide an insight into the production of glucomannan-type of polysaccharide. This low molecular weight linear form of glucomannan-type EPS might be involved to form a network-like unattached aggregation, and helps in cell-to-cell interaction in deep-sea microbial species.
biosynthesis, structure, extracellular polysaccharide, EPS, marine, deep-sea, Bacillus altitudinis
NCBI PubMed ID: 36175467Publication DOI: 10.1038/s41598-022-20822-3Journal NLM ID: 101563288Publisher: London: Nature Publishing Group
Correspondence: rajibindia@gmail.com
Institutions: Microbiology Section, Department of Botany, The University of Burdwan, Burdwan, West Bengal, 713104, India, National Agri-Food Biotechnology Institute, Sector 81, SAS Nagar, Punjab, 140308, India, Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, 835215, India
Methods: 13C NMR, 1H NMR, methylation, IR, GC-MS, chemical analysis, composition analysis, GPC, SEM, thermal analysis, FESEM
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