Show legend Show as text

Structure type: oligomer
Compound class: LPS, core oligosaccharide with O-unit
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_135394,IEDB_136105,IEDB_137340,IEDB_137473,IEDB_140088,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_150908,IEDB_151531,IEDB_189517,IEDB_2189047,IEDB_225177,IEDB_885823,IEDB_983931,SB_192

• Article ID: 501
  Bystrova OV, Lindner B, Moll H, Kocharova NA, Shashkov AS, Knirel YA, Zähringer U, Pier GB "Full structure of the lipopolysaccharide of Pseudomonas aeruginosa immunotype 5" - Biochemistry (Moscow) 69(2) (2004) 170-175
  

  The lipopolysaccharide (LPS) of the opportunistic human pathogen Pseudomonas aeruginosa immunotype 5 was delipidated by mild acid hydrolysis, and the products were separated by high-performance anion-exchange chromatography and analyzed by ESI MS and NMR spectroscopy. LPS species of three types were found, including those with an unsubstituted core and the core substituted with one O-polysaccharide repeating unit or with an O-polysaccharide of a variable number of repeating units. The core region is highly phosphorylated, the major species containing two monophosphate groups and one ethanolamine diphosphate group. Based on these and published data on the O-polysaccharide structure, the full structure of the LPS of P. aeruginosa immunotype 5 was established.

  Lipopolysaccharide, O-antigen, repeating unit, Pseudomonas aeruginosa, core oligosaccharide, ethanolamine diphosphate

  NCBI PubMed ID: 15000683
  Journal NLM ID: 0376536
  Publisher: Nauka/Interperiodica
  Correspondence: knirel@ioc.ac.ru
  Institutions: Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany, Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, USA
  Methods: NMR-2D, NMR, ESI-MS, HPAEC
  
   
  
  Pseudomonas aeruginosa 5
  CSDB ID 9222 (all data & tools)
• Article ID: 3208
  Bystrova OV, Knirel YA, Lindner B, Kocharova NA, Kondakova AN, Zähringer U, Pier GB "Structures of the core oligosaccharide and O-units in the R- and SR-type lipopolysaccharides of reference strains of Pseudomonas aeruginosa O-serogroups" - FEMS Immunology and Medical Microbiology 46(1) (2006) 85-99
  

  Highly phosphorylated core oligosaccharides and those substituted with one O-antigen repeating unit were obtained by mild acid degradation or strong alkaline hydrolysis of lipopolysaccharide samples from 23 reference strains representing all Pseudomonas aeruginosa O-serogroups. Studies by high-resolution electrospray ionization mass spectrometry and two-dimensional NMR spectroscopy revealed both conserved and variable structural features of the lipopolysaccharides of various O-serogroups. The upstream terminal saccharide of the O-antigen, which contributes most to the immunospecificity of the bacteria, was defined in 11 from a total of 13 O-serogroups. The data obtained link together the known biosynthesis pathways, genetics and serology of the P. aeruginosa lipopolysaccharide.

  lipopolysaccharides, oligosaccharide, structure, core, strain, O-antigen, Pseudomonas, Pseudomonas aeruginosa, core oligosaccharide, reference, lipopolysaccharide structure, preparation, PDF, O-unit

  NCBI PubMed ID: 16420601
  Publication DOI: 10.1111/j.1574-695X.2005.00004.x
  Journal NLM ID: 9315554
  Publisher: Elsevier
  Correspondence: olgabv@ioc.ac.ru
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany, Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston,MA, USA
  Methods: NMR-2D, mild acid hydrolysis, alkaline degradation, ESI-ICR-MS
  
   
  
  Pseudomonas aeruginosa O10
  CSDB ID 20594 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: core oligosaccharide with O-unit
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_135394,IEDB_136105,IEDB_137340,IEDB_137473,IEDB_140088,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_150908,IEDB_151531,IEDB_189517,IEDB_2189047,IEDB_225177,IEDB_885823,IEDB_983931,SB_192

• Article ID: 3208
  Bystrova OV, Knirel YA, Lindner B, Kocharova NA, Kondakova AN, Zähringer U, Pier GB "Structures of the core oligosaccharide and O-units in the R- and SR-type lipopolysaccharides of reference strains of Pseudomonas aeruginosa O-serogroups" - FEMS Immunology and Medical Microbiology 46(1) (2006) 85-99
  

  Highly phosphorylated core oligosaccharides and those substituted with one O-antigen repeating unit were obtained by mild acid degradation or strong alkaline hydrolysis of lipopolysaccharide samples from 23 reference strains representing all Pseudomonas aeruginosa O-serogroups. Studies by high-resolution electrospray ionization mass spectrometry and two-dimensional NMR spectroscopy revealed both conserved and variable structural features of the lipopolysaccharides of various O-serogroups. The upstream terminal saccharide of the O-antigen, which contributes most to the immunospecificity of the bacteria, was defined in 11 from a total of 13 O-serogroups. The data obtained link together the known biosynthesis pathways, genetics and serology of the P. aeruginosa lipopolysaccharide.

  lipopolysaccharides, oligosaccharide, structure, core, strain, O-antigen, Pseudomonas, Pseudomonas aeruginosa, core oligosaccharide, reference, lipopolysaccharide structure, preparation, PDF, O-unit

  NCBI PubMed ID: 16420601
  Publication DOI: 10.1111/j.1574-695X.2005.00004.x
  Journal NLM ID: 9315554
  Publisher: Elsevier
  Correspondence: olgabv@ioc.ac.ru
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany, Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston,MA, USA
  Methods: NMR-2D, mild acid hydrolysis, alkaline degradation, ESI-ICR-MS
  
   
  
  Pseudomonas aeruginosa O3a,3b,3c
  CSDB ID 20595 (all data & tools)