Structural studies on the major glycolipid isolated from Rothia mucilaginosa were carried out utilising specific chemical degradation, NMR spectroscopy and matrix-assisted laser-desorption/ionization time of flight mass spectrometry (MALDI TOF-MS). The glycolipid was found to be a dimannosylacylmonoglyceride in which the carbohydrate part was the glycerol-linked dimannoside α-D-Manp-(1→3)-α-D-Manp-(1→3)-sn-Gro (Man A-Man B-Gro), of which Man B was esterified at O-6 by a fatty acid residue. A second fatty acid substituted the secondary methylene position of the glycerol residue, in contrast to the glycolipid previously found in R. dentocariosa and Saccharopolyspora strains, in which the second fatty acid esterified the primary methylene position of glycerol. Results of the ELISA experiment with rabbit specific antibacterial sera indicate that these two major glycolipids are antigenic, and the patterns of serological reactivity are similar but not identical.
NMR, structure, glycolipid, Rothia mucilaginosa, Stomatococcus, Opportunistic agent, Dimannosylacylmonoglyceride
NCBI PubMed ID: 15535967Publication DOI: 10.1016/j.bbagen.2004.08.004Journal NLM ID: 0217513Publisher: Elsevier
Correspondence: gamian@immuno.iitd.pan.wroc.pl
Institutions: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland, Division of Structural Biochemistry, Research Center Borstel, Leibniz Center for Medicine and Biosciences, Germany, Division of Biophysics, Research Center Borstel, Leibniz Center for Medicine and Biosciences, Borstel, Germany
Methods: NMR-2D, NMR, MALDI-TOF MS
Found