In the present study, a cost-effective, robust Microbioreactor based production optimization of levan like exopolysaccharide from marine Bacillus sp. SGD-03 was analysed. FE-SEM analysis has showed the significant fibrillar structure of EPS. Size exclusion chromatography and other analytical data revealed that, produced EPS has a molecular weight of 1.0 × 104 Da and is composed of fructose monosaccharide with hydroxyl, carbonyl, and ether groups. The backbone structure of EPS has a branching pattern of β-(2,6) linkages which confirms the similarity with available levan like polymers. The cost-effective media composition for levan production was demonstrated. The maximum yield of crude levan obtained was 123.9 g/L by response surface methodology using robust BioLector Pro Microbioreactor, and same has been validated with shake flask, 1 L and 10 L pilot-scale fermentation.
structure, EPS, marine, optimization, microbioreactor
NCBI PubMed ID: 35287863Publication DOI: 10.1016/j.carbpol.2022.119241Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: S.G. Dastager
Institutions: Academy of Scientific and Innovative Research (AcSIR), New Delhi, India, NCIM Resource Center, CSIR-National Chemical Laboratory, Pune 411008, India, Division of Organic Chemistry, CSIR-National Chemical Laboratory, Pune 411008, India, Beckman Coulter Life Sciences, Mumbai, India
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, sugar analysis, TLC, FTIR, SEC-HPLC, SEM, DEPT, genome sequencing, UHPLC, FE-SEM, EPS production, data analysis, screening of EPS producing strain, LC-HRMS
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