Alginate that was purified from the fermentation solution of marine bacteria Vibro sp. 510 under specific reaction conditions was hydrolyzed by alginate lyase. Seven oligosaccharides, including di-, tri- and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (LP-GPC) and semipreparative strong-anion exchange (SAX) fast-protein liquid chromatography (FPLC). The oligosaccharide structures were elucidated based on ESIMS and 2D NMR spectral analysis. The hydrolytic specificity of this alginate lyase to alginate is discussed.
Oligosaccharides, alginate, alginate lyase, guluronic acid, mannuronic acid
NCBI PubMed ID: 15178390Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: glyu@mail.ouc.edu.cn
Institutions: Institute of Marine Drug and Food, Ocean University of China, Qingdao, China, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China, Department of Food Science and Technology, Ocean University of China, Qingdao, China
Methods: gel filtration, NMR-2D, enzymatic hydrolysis, ESI-MS, anion-exchange chromatography, fast protein chromatography
Found