Exopolysaccharide (EPS)-producing Bifidobacterium bifidum EPS DA-LAIM was isolated from healthy human feces, the structure of purified EPS from the strain was analyzed, and its prebiotic activity was evaluated. The EPS from B. bifidum EPS DA-LAIM is a glucomannan-type heteropolysaccharide with a molecular weight of 407-1007 kDa, and its structure comprises 2-mannosyl, 6-mannosyl, and 2,6-mannosyl residues. The purified EPS promoted the growth of representative lactic acid bacteria and bifidobacterial strains. Bifidobacterium bifidum EPS DA-LAIM increased nitric oxide production in RAW 264.7 macrophage cells, indicating its immunostimulatory activity. Bifidobacterium bifidum EPS DA-LAIM also exhibited high gastrointestinal tract tolerance, gut adhesion ability, and antioxidant activity. These results suggest that EPS from B. bifidum EPS DA-LAIM is a potentially useful prebiotic material, and B. bifidum EPS DA-LAIM could be applied as a probiotic candidate.
exopolysaccharide, Bifidobacterium bifidum, probiotics, Prebiotics, Immunostimulatory activity
NCBI PubMed ID: 36911335Publication DOI: 10.1007/s10068-022-01213-wJournal NLM ID: 9816587Publisher: Seoul, Korea: Korean Society of Food Science and Technology
Correspondence: Y.S. Park
Institutions: Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120 Republic of Korea, Research Laboratory, Dong-A Pharmaceutical Co., Ltd., Yongin, 17073 Republic of Korea
Methods: methylation, GC-MS, DNA techniques, HPAEC, composition analysis, HPSEC, statistical analysis, cytotoxicity assay, SEM, genome sequencing, acid-tolerance, bile-tolerance, pancreatin-tolerance, DPPH radical scavenging activity
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