Utilizing sugar, methylation, and absolute configurations analyses as well as NMR spectroscopy, the chemical repeating unit of the O-specific polysaccharide of Pectobacteriumversatile CFBP6051T was identified as: The polymer contains residues of an unusual, higher-branched monosaccharide, named erwiniose (3,6,8-trideoxy-4-C-(R-1-hydroxyethyl)-d-gulo-octose). Comparison of the P. versatile CFBP6051T O-polysaccharide with those isolated from strains of other Pectobacterium species indicated high differentiation in their structures within this genus.
3, higher branched monosaccharide, Pectobacterium versatile, 6, 8-Trideoxy-4-C-(R-1-hydroxyethyl)-d-gulo-octose, erwiniose, Pectinolytic bacterium
NCBI PubMed ID: 36669445Publication DOI: 10.1016/j.carres.2023.108743Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Z. Kaczyński
Institutions: Laboratory of Structural Biochemistry, Faculty of Chemistry, University of Gdansk, 63 Wita Stwosza, 80-308, Gdansk, Poland, Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk & Medical University of Gdansk, 58 Abrahama, 80-307, Gdansk, Poland
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, gel filtration, NMR-2D, sugar analysis, GLC, GC, extraction, isolation, cultivation
Found