Ochrobactrum endophyticum (syn. Brucella endophytica) is an aerobic species of Alphaproteobacteria isolated from healthy roots of Glycyrrhiza uralensis. Here we report the structure of the O-specific polysaccharide obtained by mild acid hydrolysis of the lipopolysaccharide of the type strain KCTC 42485: →3)-α-l-FucpNAc-(1→3)-β-d-QuipNAc-(1→2)-β-d-Fucp3NAcyl-(1→ where Acyl is 3-hydroxy-2,3-dimethyl-5-oxoprolyl. The structure was elucidated using chemical analyses along with 1H and 13C NMR spectroscopy (including 1H,1H COSY, TOCSY, ROESY and 1H,13C HSQC, HMBC, HSQC-TOCSY and HSQC-NOESY experiments). To our knowledge the OPS structure is novel and has not been previously published.
Lipopolysaccharide, bacterial polysaccharide structure, 3-hydroxy-2.3-dimethyl-5-oxoprolyl, Brucella endophytica, Ochrobactrum endophyticum
NCBI PubMed ID: 37040681Publication DOI: 10.1016/j.carres.2023.108810Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: E.N. Sigida
Institutions: Institute of Biochemistry and Physiology of Plants and Microorganisms, FRC Saratov Scientific Centre of Russian Academy of Sciences, 13, Prospekt Entuziastov, Saratov, 410049, Russia, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991, Moscow, Russia
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, acid hydrolysis, GLC, GPC
Found