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Structure type: oligomer
Compound class: tetrasaccharide
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137485,IEDB_140116,IEDB_141111,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_164174,IEDB_164175,IEDB_164176,IEDB_174840,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_197,SB_44,SB_67,SB_72

• Article ID: 6460
  Maruyama Y, Nakajima T, Ichishima E "A 1,2-a-D-mannosidase from a Bacillus sp.: purification, characterization, and mode of action" - Carbohydrate Research 251 (1994) 89-98
  

  A 1,2-α-D-mannosidase was purified to homogeneity from the culture supernatant of Bacillus sp. M-90, which was isolated from soil by enrichment culture on baker's yeast mannan. The purified enzyme had M(r) 380,000 Da, and was comprised of two apparently identical 190,000 Da subunits. It had a neutral optimum pH (7.0) and an isoelectric point of 3.6. The enzyme was highly specific for α-1,2-linked D-mannose oligosaccharides. An N-linked high-mannose type oligosaccharide, Man9GlcNAc2, was a good substrate, yielding Man5GlcNAc2, and the α-1,2-linked side chains of Saccharomyces cerevisiae mannan were also specifically hydrolyzed by the enzyme. p-Nitrophenyl α-D-mannopyranoside and 1,2-α-D-mannobiitol were not hydrolyzed at all. Calcium ion, 1-deoxyman-nojirimycin, and swainsonine had no effect on the enzyme, but the activity was completely inhibited by EDTA. The mode of action on α-1,2-linked mannotetraose indicated that the enzyme is an exo-1,2-α-D-mannanase.

  NCBI PubMed ID: 8149382
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai-shi, Japan
  Methods: 1H NMR, SDS-PAGE, HPLC, enzyme assay, enzymatic digestion, gel electrofocusing
  
   
  
  Saccharomyces cerevisiae
  CSDB ID 267564 (all data & tools)
• Article ID: 7654
  Shibata N, Kobayashi H, Suzuki S "Immunochemistry of pathogenic yeast, Candida species, focusing on mannan" - Proceedings of the Japan Academy. Series B, Physical and Biological Sciences 88(6) (2012) 250-265
  

  This review describes recent findings based on structural and immunochemical analyses of the cell wall mannan of Candida albicans, and other medically important Candida species. Mannan has been shown to consist of α-1,2-, α-1,3-, α-1,6-, and β-1,2-linked mannopyranose units with few phosphate groups. Each Candida species has a unique mannan structure biosynthesized by sequential collaboration between species-specific mannosyltransferases. In particular, the β-1,2-linked mannose units have been shown to comprise a characteristic oligomannosyl side chain that is strongly antigenic. For these pathogenic Candida species, cell-surface mannan was also found to participate in the adhesion to the epithelial cells, recognition by innate immune receptors and development of pathogenicity. Therefore, clarification of the precise chemical structure of Candida mannan is indispensable for understanding the mechanism of pathogenicity, and for development of new antifungal drugs and immunotherapeutic procedures.

  pathogenicity, mannan, 1H NMR, Candida, Mannosyltransferase, Dectin-2

  NCBI PubMed ID: 22728440
  Publication DOI: 10.2183/pjab.88.250
  Journal NLM ID: 9318162
  Publisher: Japan Academy
  Correspondence: suzusige@seagreen.ocn.ne.jp
  Institutions: Sendai Research Institute for Mycology, Miyagi, Japan, Department of Infection and Host Defense, Tohoku Pharmaceutical University, Miyagi, Japan, Department of Microbiology, Nagasaki International University, Nagasaki, Japan, Tohoku Pharmaceutical University, Miyagi, Japan
  
   
  
  Saccharomyces cerevisiae
  CSDB ID 45764 (all data & tools)