The structure of the K141 type capsular polysaccharide (CPS) produced by Acinetobacter baumannii KZ1106, a clinical isolate recovered from Kazakhstan in 2016, was established by sugar analyses and one- and two-dimensional 1H and 13C NMR spectroscopy. The CPS was shown to consist of branched tetrasaccharide repeating units (K-units) with the following structure: This structure was found to be consistent with the genetic content of the KL141 CPS biosynthesis gene cluster at the chromosomal K locus in the KZ1106 whole genome sequence. Assignment of the encoded enzymes allowed the first sugar of the K unit to be identified, which revealed that the β-d-GlcpNAc-(1→3)-d-GlcpNAc bond is the linkage between K-units formed by the WzyKL141 polymerase
Acinetobacter baumannii, capsular polysaccharide, K locus, K141, KL141
NCBI PubMed ID: 38555658Publication DOI: 10.1016/j.carres.2024.109097Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: j.kenyon@griffith.edu.du
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Central Scientific Research Institute of Epidemiology, Moscow, Russia, Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Russia, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia, School of Pharmacy and Medical Sciences, Health Group, Griffith University, Gold Coast, Australia
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, DNA techniques, acid hydrolysis, GLC, extraction, acetylation, reduction, dialysis, centrifugation
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