Asaia bogorensis is a Gram-negative bacterium isolated from flowers and fruits growing in tropical climate, reproductive system of mosquitoes, and rarely from immunocompromised patients. In Europe, A. bogorensis is responsible for the contamination of flavoured mineral waters. One of the important surface antigen and an element of the bacterial biofilm is lipopolysaccharide (LPS, endotoxin). To date, no data on A. bogorensis LPS structure has been reported. Chemical analysis and 1H,13C nuclear magnetic resonance spectroscopy revealed the novel structure of the O-specific polysaccharide of A. bogorensis ATCC BAA-21 LPS. It was concluded that the repeating unit of the O-antigen is a branched trisaccharide with the following structure: →6)-α-d-Glcp-(1→2)-[β-d-Glcp-(1→3)]-α-l-Rhap-(1→
Lipopolysaccharide, NMR, O-antigen, O-specific polysaccharide, Asaia bogoriensis
NCBI PubMed ID: 39260148Publication DOI: 10.1016/j.carres.2024.109266Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: anna.maciejewska@hirszfeld.pl
Institutions: Laboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland, 3Faculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Katowice, Poland
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, SDS-PAGE, acid hydrolysis, enzymatic digestion, extraction, acetylation, MALDI-TOF, methylation analysis, SEC, reduction, dialysis, derivatization, centrifugation, staining
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