An extracellular acidic polysaccharide elaborated by Acetobacter sp. NBI 1005 was composed of D-glucose, D-galactose, D-mannose, and D-glucuronic acid (approximate molar ratio, 6:2:1:1). Methylation and fragmentation analysis by partial acid hydrolysis indicated that the polysaccharide has a branched structure containing a backbone chain of β-(l→4)-linked D-glucose residues, two out of every four glucose residues being substituted at the 0-3 positions to form two kinds of branches, one consisting of D-mannose and D-glucuronic acid residues and the other of (l→6)-linked D-galactose and D-glucose residues. © 1986, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

• Compound ID: 4458
  

  b-D-GlcpA-(1-2)-D-Manp-(1-3)-+ | b-D-Glcp-(1-6)-b-D-Glcp-(1-6)-b-D-Glcp-(1-6)-D-Galp-(1-6)-D-Galp-(1-3)-+ | | | -4)-b-D-Glcp-(1-4)-b-D-Glcp-(1-4)-b-D-Glcp-(1-4)-b-D-Glcp-(1-

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  Structure type: polymer chemical repeating unit
  Compound class: EPS
  Reference(s) to other database(s): GTC:G89662PR, GlycomeDB:778
  
   
  
  Acetobacter sp. NBI 1005
  CSDB ID 101228 (all data & tools)

The usage of polysaccharides as biodegradable polymers is of growing interest in the context of a sustainable and ecofriendly economy. For this, the production of exopolysaccharides (EPS) by Gluconacetobacter sp. was investigated. Glycerol as carbon source revealed to be beneficial compared to glucose. In addition, pure glycerol could be substituted by a crude glycerol waste stream from biodiesel production. Systematic analysis of the peptone and phosphate concentrations in glycerol-based media indicated a strong effect of peptone. Optimized parameters resulted in a titer of 25.4 ± 2.4 g/L EPS with a productivity of 0.46 ± 0.04 g*(L*h)-1. With decreasing peptone, a variation in the monomer ratios was observed. An accompanying change in molecular size distribution indicated the production of two different polysaccharides. Intensified analysis revealed the main polysaccharide to be composed of glucose (Glc), galactose (Gal), mannose (Man) and glucuronic acid (GlcA), and the minor polysaccharide of Gal, Man, ribose (Rib).

exopolysaccharide, optimization

• Compound ID: 1720
  

  a-L-Rhap-(1-6)-b-D-Glcp-(1-6)-a-D-Glcp-(1-4)-b-D-GlcpA-(1-2)-a-D-Manp6Ac-(1-3)-+ | -4)-b-D-Glcp-(1-4)-b-D-Glcp6Ac-(1-

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  Structure type: polymer chemical repeating unit
  Trivial name: acetan
  Compound class: EPS
  Reference(s) to other database(s): GTC:G85208LB, GlycomeDB:27443
  
   
  
  Acetobacter sp. NBI 1005, Acetobacter xylinum NRRL-B42
  CSDB ID 9174 (all data & tools)
• Compound ID: 4458
  

  b-D-GlcpA-(1-2)-D-Manp-(1-3)-+ | b-D-Glcp-(1-6)-b-D-Glcp-(1-6)-b-D-Glcp-(1-6)-D-Galp-(1-6)-D-Galp-(1-3)-+ | | | -4)-b-D-Glcp-(1-4)-b-D-Glcp-(1-4)-b-D-Glcp-(1-4)-b-D-Glcp-(1-

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  Structure type: polymer chemical repeating unit
  Compound class: EPS
  Reference(s) to other database(s): GTC:G89662PR, GlycomeDB:778
  
   
  
  Acetobacter pasteurianus subsp. estunensis, Gluconacetobacter capsulatus IAM 1813, Acetobacter sp. NBI 1005
  CSDB ID 8537 (all data & tools)
• Compound ID: 1724
  

  b-D-GlcpA-(1-2)-a-D-Manp6Ac-(1-3)-+ | -4)-b-D-Glcp6Ac-(1-4)-b-D-Glcp-(1-

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  Structure type: polymer chemical repeating unit
  Trivial name: acetan, acetan tetramer, CR1/4
  Compound class: EPS
  Reference(s) to other database(s): GTC:G66584GU, GlycomeDB:25365
  
   
  
  Acetobacter xylinum CR1/4
  CSDB ID 9176 (all data & tools)
• Compound ID: 16274
  

  a-L-Rhap-(1-6)-b-D-Glcp-(1-6)-a-D-Glcp-(1-4)-b-D-GlcpA-(1-2)-a-D-Manp-(1-3)-+ | a-L-Rhap-(1-6)-b-D-Glcp-(1-6)-a-D-Glcp-(1-4)-b-D-GlcpA-(1-2)-a-D-Manp-(1-3)-+ | | | a-L-Rhap-(1-6)-b-D-Glcp-(1-6)-a-D-Glcp-(1-3)-+ | | | | | -6)-b-D-Glcp-(1-4)-b-D-Glcp-(1-4)-{{{-b-D-Glcp-(1-4)-b-D-Glcp-(1-4)-}}}b-D-Glcp-(1-4)-b-D-Glcp-(1-

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  Structure type: structural motif or average structure
  Compound class: EPS
  
   
  
  Gluconacetobacter xylinus CNCM I-2281
  CSDB ID 9173 (all data & tools)
• Compound ID: 1722
  

  a-D-Glcp-(1-4)-b-D-GlcpA-(1-2)-a-D-Manp6Ac-(1-3)-+ | -4)-b-D-Glcp6Ac-(1-4)-b-D-Glcp-(1-

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  Structure type: polymer chemical repeating unit
  Trivial name: acetan, P2
  Compound class: EPS
  Reference(s) to other database(s): GTC:G36740TZ, GlycomeDB:25366
  
   
  
  Acetobacter xylinum CKE5 (ΔaceP)
  CSDB ID 9175 (all data & tools)