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Structure type: oligomer
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137485,IEDB_140116,IEDB_141111,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_164174,IEDB_164176,IEDB_983930,SB_136,SB_196,SB_197,SB_44,SB_67,SB_72

• Article ID: 6717
  Ferro V, Li C, Fewings K, Palermo MC, Linhardt RJ, Toida T "Determination of the composition of the oligosaccharide phosphate fraction of Pichia (Hansenula) holstii NRRL Y-2448 phosphomannan by capillary electrophoresis and HPLC" - Carbohydrate Research 337 (2002) 139-146
  

  The promising new anticancer agent, PI-88, is prepared by the sulfonation of the oligosaccharide phosphate fraction of the extracellular phosphomannan produced by the yeast Pichia (Hansenula) holstii NRRL Y-2448. The composition of the oligosaccharide phosphate fraction was determined by capillary electrophoresis (CE) with indirect UV detection using 6 mM potassium sorbate at pH 10.3 as the background electrolyte. Further confirmation of the composition was obtained by HPLC analysis of a sample dephosphorylated by treatment with alkaline phosphatase. The structure of the hexasaccharide component has been determined by isolation and NMR spectroscopic analysis of its dephosphorylated derivative. Additionally, the structure of a second, previously undetected tetrasaccharide component (a hexosamine) has been determined by isolation and NMR spectroscopic analysis of the acetate of its dephosphorylated derivative. It is demonstrated that CE is an ideal method for the quality control of the oligosaccharide phosphate fraction for use in the production of PI-88.

  capillary electrophoresis, Pichia (Hansenula) holstii, phosphomannan, oligosaccharide phosphate fraction, PI-88

  Journal NLM ID: 0043535
  WWW link: http://www.sciencedirect.com/science/article/pii/S0008621501003007
  Publisher: Elsevier
  Correspondence: vito.ferro@progen.com.au
  Institutions: Department of Research & Development, Progen Industries Ltd, PO Box 28, Richlands BC, Qld 4077, Australia
  Methods: 13C NMR, 1H NMR, GC-MS, dephosphorylation, TLC, ESI-MS, methanolysis, HPLC, UV, methylation analysis, column chromatography, HMBC, HMQC, capillary electrophoresis, COSY, NOESY, ion chromatography
  
   
  
  Pichia holstii NRRL Y-2448
  CSDB ID 40603 (all data & tools)
• Article ID: 6722
  Parolis LA, Parolis H, Kenne L, Meldal M, Bock K "The extracellular polysaccharide of Pichia (Hansenula) holstii NRRL Y-2448: the phosphorylated side chains" - Carbohydrate Research 309 (1998) 77-87
  

  The exopolysaccharide produced by Pichia (Hansenula) holstii NRRL Y-2448 is composed of a phosphomannan core to which oligosaccharide diester phosphate side chains are appended. The oligosaccharides of the side chains were released as oligosaccharide phosphates and neutral oligosaccharides by mild hydrolysis with aqueous acetic acid and aqueous hydrogen fluoride, respectively. The liberated oligosaccharide phosphates were studied by NMR spectroscopy and by electrospray and fast atom bombardment mass spectrometry. The structures of the neutral oligosaccharides were determined by 1D and 2D NMR spectroscopic experiments. Further insight into the length of the side chains was obtained from a matrix assisted laser desorption ionisation-time of flight mass spectrometric study of high and low molecular weight fragments obtained from partial acid hydrolysis of the native polysaccharide.

  NMR spectroscopy, exopolysaccharide, phosphomannan, Pichia holstii, oligosaccharide side chains

  Publication DOI: 10.1016/S0008-6215(98)00101-3
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: School of Pharmaceutical Sciences, Rhodes University, Grahamstown 6140, South Africa, Department of Chemistry, Carlsberg Laboratory, Gamle Carlsbergvej 10, 2500 Valby, Denmark, Department of Chemistry, Swedish University of Agricultural Sciences, S-750 07 Uppsala, Sweden
  Methods: 13C NMR, 1H NMR, FAB-MS, gel permeation chromatography, HMBC, HMQC, COSY, NOESY, HOHAHA, MALDI-TOF-MS, HMQC-TOCSY, aqueous HF hydrolysis
  
   
  
  Pichia holstii NRRLY-2448
  CSDB ID 40624 (all data & tools)