Torulaspora delbrueckii starin IFO 0955 was examined with respect to its structural and serological properties of the cell wall mannan (Td-0955-M). Td-0955-M revealed significant reactivities with sera from a commercially available factor serum kit (Candida Check) in ELISA. Td-0955-M was investigated for its chemical structure by acetolysis under conventional and mild conditions. NMR and GC techniques were used as analytical techniques. The mannooligosaccharide fractions eluted from a Bio-Gel P-2 column were found to consist of Man(α1-2)Man, M2, Man(α1-2)Man(α1-2)Man and Man(β1-2)Man(α1-2)Man, M3, Man(α1-2)Man(β1-2)Man(β1-2)Man(α1-2)Man, M5, and a new mannoheptaose, which possesses the structure, Man(α1-2)Man(β1-2)Man(β1-2)Man(β1-2)Man(β1-2)Man(α1-2)Man, M7. The results of the inhibition ELISA showed that the M7 oligosaccharide significantly inhibited the reactivities in the Td-0955-M-factor serum systems.
serology, mannan, antigenic epitopes, yeast, Torulaspora delbrueckii, Manno-oligosaccharides
Publication DOI: 10.1016/S0008-6215(03)00146-0Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: okawa@tohoku-pharm.ac.jp
Institutions: Second Department of Hygienic Chemistry, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Sendai Aoba-ku, Miyagi 981-8558, Japan
Methods: 1H NMR, methylation, ELISA, GC, chemical methods, serological methods, GPC, acetolysis, GFC, 2D-HOHAHA, chemical metods
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