The structures of the core oligosaccharides of the lipopolysaccharides (LPS) from Actinobacillus pleuropneumoniae serotypes 1, 2, 5a and 5b were elucidated. The LPS's were subjected to a variety of degradative procedures. The structures of the purified products were established by monosaccharide and methylation analyses, NMR spectroscopy and mass spectrometry. The following structures for the core oligosaccharides were determined on the basis of the combined data from these experiments. For serotype 1: R is (1S)-GalaNAc-(1→4,6)-α-Gal II-(1→3)-β-Gal I-(1→, and R' is H For serotype 2: R is β-Glc III-(1→, and R' is D-α-D-Hep V-(1→ For serotypes 5a and 5b: R is H and R' is D-α-D-Hep V-(1→ All oligosaccharides elaborated a conserved inner core structure, as illustrated. All sugars were in the pyranose ring form apart from the open-chain N-acetylgalactosamine, the identification of which in the serotype 1 LPS was of interest.
NMR, LPS, core oligosaccharide, mass spectrometry, Actinobacillus pleuropneumoniae
NCBI PubMed ID: 15261591Publication DOI: 10.1016/j.carres.2004.04.019Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: andrew.cox@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, 100 Sussex Drive, Ottawa, ON, Canada K1A 0R6, Faculte de medecine veterinaire, Universite de Montreal, St-Hyacinthe, Quebec, Canada J2S-7C6
Methods: methylation, NMR-2D, NMR, sugar analysis, CE-MS/MS
Found