When grown on vegetable oils and their derivatives, the smut fungus Ustilago maydis (DSM 4500 and ATCC 14826) produces several glycolipids under nitrogen-limiting conditions. With 45 g l(-1) sunflower oil fatty acids (technical grade) a yield of 30 g l(-1) glycolipid was achieved. The resulting mixture contained predominantly mannosylerythritol lipids together with smaller amounts of cellobiose lipids. The production of the more polar cellobiose lipids was enhanced when glucose was used as carbon source. The molecular structure of the main components of the glycolipid mixture were elucidated by a combination of NMR spectroscopic and mass-spectrometric techniques.
NMR, mannosylerythritol lipids, derivatives, mannosylerythritol lipids candida-antarctica identification biosurfactants alkanes
Publication DOI: 10.1007/s002530051359Journal NLM ID: 8406612Publisher: Springer
Institutions: Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Spielmannstrasse 7, D-38106 Braunschweig, Germany, GBF (National Institute for Biotechnological Research), Mascheroder Weg 1, D-38124 Braunschweig, Germany, Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Braunschweig, Germany, GBF (National Institute for Biotechnological Research), Braunschweig, Germany
Methods: GC-MS, TLC, ESI-MS/MS, GC, HMBC, HMQC, COSY
Found