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Structure type: homopolymer
Compound class: O-polysaccharide, O-antigen

• Article ID: 37
  Caroff M, Karibian D "Structure of bacterial lipopolysaccharides" - Carbohydrate Research 338(23) (2003) 2431-2447
  

  Bacterial lipopolysaccharides are the major components of the outer surface of Gram-negative bacteria They are often of interest in medicine for their immunomodulatory properties. In small amounts they can be beneficial, but in larger amounts they may cause endotoxic shock. Although they share a common architecture, their structural details exert a strong influence on their activity. These molecules comprise: a lipid moiety, called lipid A, which is considered to be the endotoxic component, a glycosidic part consisting of a core of approximately 10 monosaccharides and, in 'smooth-type' lipopolysaccharides, a third region, named O-chain, consisting of repetitive subunits of one to eight monosaccharides responsible for much of the immunospecificity of the bacterial cell.

  Lipopolysaccharide, structure, core, lipid A, endotoxin, O-chains

  NCBI PubMed ID: 14670707
  Publication DOI: 10.1016/j.carres.2003.07.010
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: martine.carloff@bbmpc.u-psud.fr
  Institutions: Equipe Endotoxines, UMR 8619 du Centre National de la Recherche Scientifique, IBBMC, Université de Paris-Sud, F-Orsay, France
  
   
  
  Bordetella avium ATCC 5086
  CSDB ID 31484 (all data & tools)
• Article ID: 3697
  Harvey DJ "Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update for 2003-2004" - Mass Spectrometry Reviews 28(2) (2009) 273-361
  

  This review is the third update of the original review, published in 1999, on the application of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings the topic to the end of 2004. Both fundamental studies and applications are covered. The main topics include methodological developments, matrices, fragmentation of carbohydrates and applications to large polymeric carbohydrates from plants, glycans from glycoproteins and those from various glycolipids. Other topics include the use of MALDI MS to study enzymes related to carbohydrate biosynthesis and degradation, its use in industrial processes, particularly biopharmaceuticals and its use to monitor products of chemical synthesis where glycodendrimers and carbohydrate-protein complexes are highlighted

  carbohydrates, glycosyltransferases, fragmentation, MALDI, glycolipids, glycoproteins, biopharmaceuticals, glycosidases, time-of-flight

  NCBI PubMed ID: 18825656
  Publication DOI: 10.1002/mas.2019
  Journal NLM ID: 8219702
  Publisher: Wiley
  Correspondence: david.harvey@bioch.ox.ac.uk
  Institutions: Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford OX1 3QU, UK
  Methods: NMR, MALDI-TOF MS
  
   
  
  Bordetella avium ATCC 5086
  CSDB ID 24006 (all data & tools)
• Article ID: 5742
  Caroff M, Novikov A "Lipopolysaccharides: structure, function and bacterial identification" - OCL - Oilseeds and fats, Crops and Lipids 27 (2020) 31
  

  Lipopolysaccharides (LPS) are the main components of the outer membrane of Gram-negative bacteria. They are glycolipids containing a lipid moiety called lipid A, more often made of a bis-phosphorylated glucosamine disaccharide, carrying fatty acids in ester and amide linkages. Lipid A is linked to a core oligosaccharide of about 10 sugars, substituted in the wild-type strains, by long-chain oligosaccharide repetitive units, extending outside the bacteria and representing their main antigens. In addition to determine the serotype of the bacterium, LPS are highly potent biological molecules, capable of eliciting at the level of minute amounts, beneficial, as well as deleterious activities.

  Lipopolysaccharide, serology, endotoxins, inflammation, structure-activity

  Publication DOI: 10.1051/ocl/2020025
  Publisher: France: EDP Sciences (ISSN: 22726977, 22576614)
  Correspondence: martine.caroff@lpsbiosciences.com
  Institutions: LPS-BioSciences, Paris-Saclay University, Orsay, France, Hephaistos-Pharma, Paris-Saclay University, Orsay, France
  
   
  
  Bordetella avium ATCC5086
  CSDB ID 3311 (all data & tools)

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Structure type: homopolymer
Compound class: O-polysaccharide

• Article ID: 920
  Larocque S, Brisson JR, Therisod H, Perry MB, Caroff M "Structural characterization of the O-chain polysaccharide isolated from Bordetella avium ATCC5086: variation on a theme" - FEBS Letters 535(1-3) (2003) 11-16
  

  The O-chain polysaccharide (O-PS) of Bordetella avium was isolated from the lipopolysaccharide by mild acid hydrolysis to remove the lipid A, followed by hydrofluorolysis to remove the lipopolysaccharide core oligosaccharide leaving a residual O-PS for structural analysis. High resolution (1)H and (13)C NMR and MALDI studies showed the O-chain to be a polymer composed of 1,4-linked 2-acetamidino-3-[3- hydroxybutanamido]-2,3-dideoxy-β-D-glucopyranosyluronic acid residues

  Lipopolysaccharide, NMR, oligosaccharide, core, structural, variation, characterization, polysaccharide, analysis, polymer, acid, lipid, lipid A, core oligosaccharide, high, lipopolysaccharide core, lipopolysaccharide core oligosaccharide, hydrolysis, Bordetella, biological, structural analysis, MALDI, O-chain, high-resolution, high resolution, resolution

  NCBI PubMed ID: 12560070
  Journal NLM ID: 0155157
  Publisher: Elsevier
  Correspondence: martine.caroff@bbmpc.u-psud.fr
  Institutions: Institute for Biological Sciences, NRC, Ottawa, ON, Canada
  Methods: NMR-2D, NMR, HF solvolysis, SDS-PAGE, sugar analysis, TLC, mild acid hydrolysis, MS
  
   
  
  Bordetella avium ATCC 5086
  CSDB ID 3116 (all data & tools)
• Article ID: 3821
  Banoub JH, El Aneed A, Cohen AM, Joly N "Structural investigation of bacterial lipopolysaccharides by mass spectrometry and tandem mass spectrometry" - Mass Spectrometry Reviews 29(4) (2010) 606-650
  

  Mass spectrometric studies are now playing a leading role in the elucidation of lipopolysaccharide (LPS) structures through the characterization of antigenic polysaccharides, core oligosaccharides and lipid A components including LPS genetic modifications. The conventional MS and MS/MS analyses together with CID fragmentation provide additional structural information complementary to the previous analytical experiments, and thus contribute to an integrated strategy for the simultaneous characterization and correct sequencing of the carbohydrate moiety.

  LPS, O-antigen, lipid A, core oligosaccharide, MS and MS/MS analyses

  NCBI PubMed ID: 20589944
  Publication DOI: 10.1002/mas.20258
  Journal NLM ID: 8219702
  Publisher: Wiley
  Correspondence: joe.banoub@dfo-mpo.gc.ca
  Institutions: Fisheries and Oceans Canada, Science Branch, Special Projects, P.O. Box 5667, St. John's, Newfoundland, Canada A1C 5X1, Department of Chemistry, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1B 3V6, College of Pharmacy and Nutrition, University of Saskatchewan, Thorvaldson Building, 110 Science Place, Saskatoon, Saskatchewan, Canada S7N 5C9, Unité de Catalyse et de Chimie du Solide, Site de l'Artois—UMR CNRS 8181, I.U.T. de Béthune, Département Chimie, 1230 rue de l'Université, BP819, 62408 Béthune Cedex, France, Institute for Marine Biosciences Room 219A, (NRC-IMB), National Research Council of Canada, Government of Canada, 1411 Oxford Street, Halifax, NS, Canada B3H 3Z1
  Methods: MS/MS, MS
  
   
  
  Bordetella avium ATCC 5086
  CSDB ID 25517 (all data & tools)
• Article ID: 4329
  Knirel YA "Structure of O-antigens" - Book: Bacterial lipopolysaccharides: Structure, chemical synthesis, biogenesis and interaction with host cells (2011) Chapter 3, 41-115
  

  The lipopolysaccharide (LPS) is the major constituent of the outer leaflet of the outer membrane of Gram-negative bacteria. Its lipid A moiety is embedded in the membrane and serves as an anchor for the rest of the LPS molecule. The outermost repetitive glycan region of the LPS is linked to the lipid A through a core oligosaccharide (OS), and is designated as the O-specific polysaccharide (O-polysaccharide, OPS) or O-antigen. The O-antigen is the most variable portion of the LPS and provides serological specificity, which is used for bacterial serotyping. The OPS also provides protection to the microorganisms from host defenses such as complement mediated killing and phagocytosis, and is involved in interactions of bacteria with plants and bacteriophages. Studies of the OPSs ranging from the elucidation of their chemical structures and conformations to their biological and physico-chemical properties help improving classification schemes of Gram-negative bacteria. Furthermore, these studies contributed to a better understanding of the mechanisms of pathogenesis of infectious diseases, as well as provided information to develop novel vaccines and diagnostic reagents.

  Lipopolysaccharide, synthesis, lipopolysaccharides, structure, Bacterial, host, O-antigen, O antigen, cell, O antigens, O-antigens, chemical, interaction, cells, PDF, chemical synthesis, biogenesis

  Publication DOI: 10.1007/978-3-7091-0733-1_3
  Publisher: Springer
  Correspondence: knirel@ioc.ac.ru
  Editors: Knirel YA, Valvano MA
  Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
  
   
  
  Bordetella avium
  CSDB ID 27894 (all data & tools)