The alkali extractable and water-soluble cell wall polysaccharides F1SS from Aspergillus wentii and Chaetosartorya chrysella have been studied by methylation analysis, 1D- and 2D-NMR, and MALDI-TOF analysis. Their structures are almost identical, corresponding to the following repeating unit: [→3)-β-D-Galf-(1→5)-β-D-Galf-(1→](n)→mannan core. The structure of this galactofuranose side chain differs from that found in the pathogenic fungus Aspergillus fumigatus, in other Aspergillii and members of Trichocomaceae: [→5)-β-D-Galf-(1→](n)→mannan core. The mannan cores have also been investigated, and are constituted by a (1→6)-α-mannan backbone, substituted at positions 2 by chains from 1 to 7 residues of (1→2) linked α-mannopyranoses.
NMR spectroscopy, polysaccharides, Aspergillus, fungi, Chaetosartorya
NCBI PubMed ID: 15115908Publication DOI: 10.1023/B:GLYC.0000025818.83019.e4Journal NLM ID: 8603310Publisher: Kluwer Academic Publishers
Correspondence: Manuel Bernabé
Institutions: Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006-Madrid, Spain, Departamento de Química Orgánica Biológica, Instituto de Química Orgánica, CSIC, Juan de la Cierva 3, 28006, Madrid, Spain
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, partial acid hydrolysis, GC-MS, sugar analysis, acid hydrolysis, GLC, MALDI-TOF MS, acetolysis, reductive cleavage, alkaline extraction
Found