Fucogalactans from Agaricus brasiliensis (EPF-Ab) and A. bisporus var. hortensis (EPF-Ah) were prepared via by aqueous extraction and a purification procedure. EPF-Ab had Mw 19.4 × 103 g/mol and EPF-Ah Mw 31.1 × 103 g/mol. EPF-Ab had a (1→6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp. EPF-Ah had a similar main-chain with O-2 substitution, but was partially methylated at HO-3, as well as having 2.5% non-reducing end-units of β-D-Gal. In mice, EPF-Ab gave 39% antinociceptive inhibition (ID50 > 100 mg/kg) and no anti-inflammatory activity. EPF-Ah also gave an inhibition of 39% at ID50 0.33 mg/kg and also inhibited by 61% (ID50 5.0 mg/kg) total cell migration and by 32% peritoneal capillary permeability, which is related to the anti-inflammatory effect. The small differences in chemical structure in these polysaccharides thus modified their biological activities.
NMR spectroscopy, Antinociceptive, Agaricus spp., Fucogalactans, Anti-inflamatory
NCBI PubMed ID: 20363124Publication DOI: 10.1016/j.biortech.2010.01.142Journal NLM ID: 9889523Publisher: Elsevier
Correspondence: Iacomini Marcello
Institutions: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Brazil, Departamento de Química, Universidade Federal de Goiás, Campus Catalão, Catalão, Brazil, Departamento de Farmacologia, Universidade Federal do Paraná, Curitiba, Brazil, Departamento de Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Florianópolis, Brazil, Departamento de Ciências Fisiológicas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Florianópolis, Brazil
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, acid hydrolysis, biological assays, composition analysis, HPSEC, extraction, reduction with NaBD4, methylation analysis, acetylation analysis
Found