The alkali-extractable and water-soluble fungal polysaccharide F1SS isolated from the cell wall of Acrospermum compressum has been studied by methylation analyses, reductive cleavage and 1D- and 2D-NMR spectroscopy. The polysaccharide consists of a regular disaccharide repeating unit with the structure: [structure: see text]. The mannan core was obtained by mild hydrolysis of the polysaccharide F1SS and its structure was deduced to be composed of a skeleton of α-(1→6)-mannopyranan, with around 1 out of 11 residues substituted at position 2 by short chains (one to six units) of 2-substituted mannopyranoses. DOSY experiments provided molecular sizes of 60 kDa and 2.5 kDa for the polysaccharide F1SS and the Mannan core, respectively. This is the first report of a fungal mannofuranose-containing cell wall polysaccharide.
NMR spectroscopy, cell wall polysaccharide, fungi, Acrospermaceae, mannofuranose
NCBI PubMed ID: 17473982Publication DOI: 10.1007/s10719-007-9032-5Journal NLM ID: 8603310Publisher: Kluwer Academic Publishers
Correspondence: Manuel Bernabé
Institutions: Centro de Investigaciones Biológicas. CSIC, Ciudad Universitaria, Madrid, Spain, Departamento de Química Orgánica Biológica, Instituto de Química Orgánica. CSIC, Madrid, Spain
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, partial acid hydrolysis, GC-MS, acid hydrolysis, GLC, GC, composition analysis, extraction, reductive cleavage
Found