On the basis of sugar analysis and NMR spectroscopy, including selective spin-decoupling, one-dimensional NOE, two-dimensional homonuclear and 13C,1H-heteronuclear correlation spectroscopy, the following structure of the acidic O-specific polysaccharide of Proteus mirabilis O43 was established: →4)-α-D-GalpA-(1→3)-α-D-GalpA-(1→3)-α-D-GlcpNAc-(1→4)-α - D-Glcp-(1→, where GalA is galacturonic acid and Galp is galactopyranose. No serological cross-reactivity was observed between lipopolysaccharides of P. mirabilis O43 and other studied Proteus strains, except for P. mirabilis O10. The O-specific polysaccharide of P. mirabilis O43 was serologically active in precipitation and inhibition tests but the activity was lost after periodate oxidation. These data suggest that the O43 specificity is determined by a wide epitope with the immunodominant role of 4-substituted D-Glc or/and D-GalA, which are destroyed by periodate oxidation
Lipopolysaccharide, LPS, structure, polysaccharide, O-antigen, O antigen, Proteus, Proteus mirabilis, serological, serology, specificity
NCBI PubMed ID: 7556207Publication DOI: 10.1111/j.1432-1033.1995.558zz.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Center of Microbiology and Virology, Polish Academy of Sciences, Lodz
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis
Found