A glucocerebroside was isolated from the n-alkane-assimilating yeast Candida deformans MIL 4041 and named “Deformin”. The cerebroside contained only a single sugar, glucose, attached to ceramide in a β-configuration, and only a single fatty acid, 2-hydroxypalmitic acid. The long-chain base was identified as (5E,9Z)-C19-sphinga-5,9-diene. Therefore, the structure of the cerebroside was determined to be (5E,9Z)-N-2′-hydroxypalmitoyl-1-O-β-glucopyranosyl-2-amino-5,9- nonadecadiene-1,3-diol. Deformin showed the fruiting-inducing activity of fungi.
glycosphingolipid, cerebroside
Publication DOI: 10.1016/0922-338X(94)90366-2Journal NLM ID: 9000935Institutions: Department of Applied Biological Science, Science University of Tokyo, Noda, Japan
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, IR, TLC, GLC, MALDI-TOF MS, NMR-1D, extraction, CC
Found