Chromatographic purification of the extract of an endophytic fungal culture yielded depsitinuside (1), a new phenolic ester together with ergosterol (2) and (22E,24S)-24-methyl-5-α-cholesta-7,22-diene-3β,5,6β-triol (3). The structure of 1 was elucidated based on 1D, 2D NMR spectroscopy and high-resolution mass spectrometry, whereas the known compounds (2 and 3) were identified by (1)H NMR, mass spectrometry, and in comparison with the literature values. Compound 1 was evaluated for its enzyme inhibitory potential against acetylcholinesterase, butyrylcholinesterase and lipoxygenase, and was found inactive (10%-40% inhibition at a concentration of 2 mg/ml).
endophytic fungus, Viburnum tinus, depside, steroids, enzyme inhibition
NCBI PubMed ID: 21985077Publication DOI: 10.1080/10286020.2011.606811Journal NLM ID: 100888334Publisher: Harwood Academic Publishers; London: Informa Healthcare
Correspondence: Saleem M
; Saleem M
Institutions: Department of Chemistry, Baghdad-ul-Jadeed Campus, The Islamia University of Bahawalpur, Bahawalpur, Pakistan, Department of Chemistry, University of Paderborn, Paderborn, Germany, Institute of Microbiology, University of Braunschweig, Braunschweig, Germany, Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Methods: 13C NMR, 1H NMR, NMR-2D, IR, TLC, GC, extraction, optical rotation measurement, CC, HR-FAB-MS
Found