Five novel sesquiterpene glycosides, namely, cosmosporasides A-E (1-5), were isolated from the ethyl acetate extract of the fermented broth of Cosmospora joca. The structures of 1-5 were elucidated on the basis of spectroscopic data analyses, monosaccharide composition analyses, and monosaccharide chirality analyses. The relative configuration of the sesquiterpene moiety of 3-5 was determined by J-based configuration analyses and supported by NOESY assignments. The inhibitory effects of 1-5 on the nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells were evaluated; all except 3 inhibited NO production at 100 μM. 4 was the most potent, with an average maximum inhibition and a median inhibitory concentration value of 71.70 ± 1.23% and 65.98 ± 0.53 μM, respectively.
sesquiterpene glycoside
NCBI PubMed ID: 21682264Publication DOI: 10.1021/np200056eJournal NLM ID: 7906882Publisher: American Society of Pharmacognosy
Correspondence: thlee@tmu.edu.tw
Institutions: Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, China, National Research Institute of Chinese Medicine, Taipei, China, Research Center, China Medical University Hospital, Taichung, Taiwan, China, Genomics Research Center, Academia Sinica, Taipei, China, Institute of Plant and Microbial Biology, Academia Sinica, Taipei, China
Methods: 13C NMR, 1H NMR, NMR-2D, IR, TLC, acid hydrolysis, HPLC, ion-exchange chromatography, extraction, optical rotation measurement, CC, ESI-TOF-MS, determination of NO production
Found