Found 1 structure.
Displayed structure 1
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1. Compound ID: 18624
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a-D-Manp-(1-2)-a-D-Manp-(1-6)-+
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a-D-Manp-(1-2)-a-D-Manp-(1-3)-a-D-Manp-(1-6)-+
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a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Glcp-(1-3)-a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)-b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)-b-D-GlcpNAc1N-(1--/pyridylamine/ |
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Structure type: oligomer
Aglycon: pyridylamine
Contained glycoepitopes: IEDB_130701,IEDB_135813,IEDB_136104,IEDB_137340,IEDB_137485,IEDB_140116,IEDB_141793,IEDB_141807,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_142488,IEDB_143632,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151079,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_164174,IEDB_232584,IEDB_429156,IEDB_857734,IEDB_983930,IEDB_983931,SB_136,SB_191,SB_192,SB_196,SB_197,SB_198,SB_44,SB_53,SB_67,SB_72,SB_73,SB_77
The structure is contained in the following publication(s):
- Article ID: 7310
Miyazaki T, Matsumoto Y, Matsuda K, Kurakata Y, Matsuo I, Ito Y, Nishikawa A, Tonozuka T "Heterologous expression and characterization of processing α-glucosidase I from Aspergillus brasiliensis ATCC 9642" -
Glycoconjugate Journal 28(8-9) (2011) 563–571
A gene for processing α-glucosidase I from a filamentous fungus, Aspergillus brasiliensis (formerly called Aspergillus niger) ATCC 9642 was cloned and fused to a glutathione S-transferase tag. The active construct with the highest production level was a truncation mutant deleting the first 16 residues of the hydrophobic N-terminal domain. This fusion enzyme hydrolyzed pyridylaminated (PA-) oligosaccharides Glc(3)Man(9)GlcNAc(2)-PA and Glc(3)Man(4)-PA and the products were identified as Glc(2)Man(9)GlcNAc(2)-PA and Glc(2)Man(4)-PA, respectively. Saturation curves were obtained for both Glc(3)Man(9)GlcNAc(2)-PA and Glc(3)Man(4)-PA, and the K (m) values for both substrates were estimated in the micromolar range. When 1 μM Glc(3)Man(4)-PA was used as a substrate, the inhibitors kojibiose and 1-deoxynojirimycin had similar effects on the enzyme; at 20 μM concentration, both inhibitors reduced activity by 50%.
N-Linked oligosaccharide, Aspergillus brasiliensis, Processing α-glucosidase I, Pyridylaminated oligosaccharide, Kojibiose
NCBI PubMed ID: 22020441Publication DOI: 10.1007/s10719-011-9356-zJournal NLM ID: 8603310Publisher: Kluwer Academic Publishers
Correspondence: Tonozuka T
Institutions: Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan, Department of Chemistry and Chemical Biology, Gunma University, Gunma, Japan, RIKEN Advanced Science Institute, Saitama, Japan
Methods: kinetics assays, HPLC, enzymatic digestion
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