Microbial transformation of cardamonin by Mucor spinosus (CGMCC 3.3450) in preparative scale resulted in the isolation of two new products. Their structures were elucidated unambiguously by ESI-MS, 1H NMR, 13C NMR and 2D NMR spectra analyses as 4-O-β-D-glucopyranosyl-6- hydroxy-2-methoxychalcone (1, 4-GluC) and 6-O-β-D-glucopyranosyl-4-hydroxy- 2-methoxychalcone (2, 6-GluC), respectively. The time-course of biotransformation by M. spinosus showed that both 4-GluC and 6-GluC appeared on the 2nd day. The optimal biotransformation temperature was 28 °C, the optimal biotransformation time was 72 h and the optimal concentration for cardamonin was 40 mg·mL-1. This is the first time for successful microbial glycosylation of cardamonin in present research.
glycosylation, Mucor spinosus, cardamonin
NCBI PubMed ID: 21882537Journal NLM ID: 21710340RPublisher: Beijing: Zhongguo yao xue hui
Correspondence: wangzht@hotmail.com
Institutions: Department of Pharmacognosy, China Pharmaceutical University, Nanjing, China, The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China, The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China
Methods: 13C NMR, 1H NMR, NMR-2D, ESI-MS, NMR-1D, HPLC, extraction, CC
Found