Lipo-oligosaccharide from phenol-water extraction of cells of Campylobacter lari strain PC 637 was separated as a water-insoluble gel of low relative molecular mass (Mr) from a water-soluble extracellular polysaccharide of high Mr. Structural investigations were performed on the lipo-oligosaccharide and the extracellular polysaccharide, variously using 1H, 13C, and 31P NMR spectroscopy, linkage analysis, and fast atom bombardment-mass spectrometry of permethylated derivatives of the glycans and their products of chemical and enzymic degradation. The following structures are proposed for the highly branched oligosaccharide region: aDGlcp(1-3)[Ac(1-2)]bDGalpN(1-3)[Ac(1-2)]bDGalpN(1-3)[aDGalp(1-2)]bDGalp(1-3)[aDGalp(1-2)]aXLDmanHepp(1-3)[bDGlcp(1-4),aDGalp(1-2)]aXLDmanHepp(1-5)?XKdo; and for the tetraglycosyl phosphate repeating unit of the extracellular polysaccharide: -P-4)bDGlcp(1-5)[aL6dgalHepp(1-2)aL6dgalHepp(1-2)]aL6dgalHepf(1-
core, LOS, polysaccharide, phosphate, extracellular polysaccharide, extracellular, lipo-oligosaccharide, 6-deoxy-L-galacto-heptose, Campylobacter lari, tetraglycosyl, phosphate polymer, tetraglycosylphosphate polymer
NCBI PubMed ID: 8593625Publication DOI: 10.1016/0008-6215(95)00283-9Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Chemistry, York University, North York, Toronto, Ontario M3J IP 3, Canada, Carbohydrate Research Centre, Department of Molecular Biology and Medical Genetics, University of Toronto, Toronto, Ontario M5S 1A5, Canada, Department of Microbiology, University of Toronto, Toronto, Ontario M5S 1A5, Canada
Methods: GLC-MS, NMR-2D, SDS-PAGE, GLC
Found