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1. (Article ID: 19)
Borrelli S, Hegedus O, Shaw DH, Jansson P, Lindberg AA
The tetrasaccharide L-a-D-heptose1->2-L-a-D-heptose1->3-L-a-D-heptose1->(3-deoxy-D-manno-octulosonic acid) and phosphate in lipid A define the conserved epitope in Haemophilus lipopolysaccharides recognized by a monoclonal antibody
Infection and Immunity 63 (1995)
3683-3692
A murine monoclonal antibody, MAHI 3 (immunoglobulin G2b), that is broadly reactive with Haemophilus influenzae lipopolysaccharides (LPSs) but nonreactive with all enterobacterial LPSs tested was generated by fusing mouse myeloma cells with spleen cells of BALB/c mice immunized with azide-killed H. influenzae RM.7004. MAHI 3 bound to all H. influenzae, all other human Haemophilus spp., all Bordetella pertussis and Bordetella parapertussis, and all Aeromonas spp. tested but not to any Neisseria or Moraxella catarrhalis strains, as determined by enzyme immunoassay, colony dot immunoblotting, and immunoblotting. In an inhibition enzyme immunoassay, MAHI 3 reacted with all 45 H. influenzae LPSs tested but not with the LPS from the rough mutant I69 Rd-/b+, which has only 3-deoxy-D-manno-octulosonic acid (P) [Kdop(P)] and lipid A. The antibody was not inhibited by H. influenzae lipid A or lipid-free polysaccharide isolated after mild acid hydrolysis. Only native LPSs show positive inhibitory activity, indicating that part of lipid A is involved in the binding of MAHI 3. From the results, it is indicated that the structural element recognized by MAHI 3 is Hep α1→2 Hep α1→3 Hep α1→ Kdo together with part of lipid A, including the phosphate
Lipopolysaccharide, Haemophilus, L-glycero-D-manno-heptose, lipopolysaccharides, LPS, core, tetrasaccharide, acid, Kdo, antibodies, antibody, conserved, epitope, lipid, lipid A, monoclonal, monoclonal antibodies, monoclonal antibody, phosphate, recognition
NCBI PubMed ID: 7543887Journal NLM ID: 0246127Publisher: American Society for Microbiology
Correspondence: Pererk.Jansson
kfc.m13.hs.sll.se
Institutions: Department of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institute, Huddinge, Sweden
Methods: de-O-acylation, SDS-PAGE, alkaline de-O-N-acylation, dephosphorylation, chemical analysis, EIA, inhibition studies, dot immunoblotting
The publication contains the following compound(s):
- Compound ID: 90
|
?%b-D-Glcp-(1-4)-b-D-Glcp-(1-4)-+
|
EtN-(1--P--6)--+ | P-4)-+
| | |
b-D-Galp-(1-2)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-Kdop-(2--/lipid A/ |
Show graphically |
Structure type: oligomer
Aglycon: lipid A
Compound class: LPS
Reference(s) to other database(s): GTC:G46164OJ
- Compound ID: 91
|
b-D-Galp-(1-4)-b-D-Glcp-(1-4)-+
|
EtN-(1--P--6)--+ | P-4)-+
| | |
EtN-(1--P--6)--L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-Kdop-(2--/lipid A/ |
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Structure type: oligomer
Aglycon: lipid A
Compound class: LPS
Reference(s) to other database(s): GTC:G74527CH
- Compound ID: 92
|
b-D-Galp-(1-4)-b-D-Glcp-(1-4)-+ P-4)-+
| |
L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-Kdop-(2--/lipid A/ |
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Structure type: oligomer
Aglycon: lipid A
Compound class: LPS
Reference(s) to other database(s): GTC:G04927WN, GlycomeDB:
25479
- Compound ID: 93
|
L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+ P-4)-+
| |
b-D-Galp-(1-4)-b-D-GlcpNAc-(1-3)-D-gro-b-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-Kdop-(2--/lipid A/ |
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Structure type: oligomer
Aglycon: lipid A
Compound class: LPS
Reference(s) to other database(s): GTC:G16400QU, GlycomeDB:
25480
- Compound ID: 94
|
a-D-Quip3NAc-(1-3)-a-D-Galp-(1-3)-+
|
a-D-Glcp-(1-4)-+ |
| |
a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-4)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-6)-Kdo
|
a-D-Glcp-(1-6)-+ |
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Structure type: oligomer
Compound class: LPS
Reference(s) to other database(s): GTC:G76753WQ, GlycomeDB:
37664
- Compound ID: 95
|
L-gro-a-D-manHepp-(1-6)-+
|
a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-6)-Kdo
|
b-D-Glcp-(1-4)-+ |
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Structure type: oligomer
Compound class: LPS
Reference(s) to other database(s): GTC:G92391SQ, GlycomeDB:
37665
- Compound ID: 96
|
a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-3)-+
|
b-D-Galp-(1-4)-+ |
| |
D-gro-a-D-manHepp-(1-6)-L-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-6)-Kdo
|
L-gro-a-D-manHepp-(1-6)-+ |
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Structure type: oligomer
Compound class: LPS
Reference(s) to other database(s): GTC:G59318UI, GlycomeDB:
37666
- Compound ID: 97
|
L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-6)-Kdo |
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Structure type: oligomer
Reference(s) to other database(s): GTC:G16640IR, GlycomeDB:
37667
- Compound ID: 98
|
a-D-GlcpN-(1-7)-L-gro-b-D-manHepp-(1-4)-+
|
b-D-Galp-(1-4)-a-D-GalpNAc-(1-6)-a-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-6)-Kdo
|
L-gro-a-D-manHepp-(1-6)-+ |
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Structure type: oligomer
Compound class: LPS
Reference(s) to other database(s): GTC:G99787IW, GlycomeDB:
37668
- Compound ID: 99
|
a-D-GlcpN-(1-7)-+ b-D-Glcp-(1-4)-+ P-4)-+
| | |
a-D-GlcpA-(1-2)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-Kdop-(2--/lipid A/ |
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Structure type: oligomer
Aglycon: lipid A
Reference(s) to other database(s): GTC:G80440SU, GlycomeDB:
25486
- Compound ID: 100
|
L-gro-a-D-manHepp-(1-7)-+
|
a-D-Galp-(1-6)-+ | a-Kdop-(2-4)-+
| | |
a-D-GlcpNAc-(1-2)-a-D-Glcp-(1-2)-a-D-Galp-(1-3)-a-D-Galp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/ |
Show graphically |
Structure type: oligomer
Aglycon: lipid A
Compound class: LPS
Reference(s) to other database(s): GTC:G83581VA, GlycomeDB:
25487
- Compound ID: 101
|
L-gro-a-D-manHepp-(1-7)-+
|
a-D-Galp-(1-6)-+ | a-Kdop-(2-4)-+
| | |
a-D-Glcp-(1-2)-a-D-Galp-(1-3)-a-D-Galp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/ |
Show graphically |
Structure type: oligomer
Aglycon: lipid A
Reference(s) to other database(s): GTC:G68203EL, GlycomeDB:
25488
- Compound ID: 102
|
L-gro-a-D-manHepp-(1-7)-+
|
a-D-Galp-(1-6)-+ | a-Kdop-(2-4)-+
| | |
a-D-Galp-(1-3)-a-D-Galp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/ |
Show graphically |
Structure type: oligomer
Aglycon: lipid A
Reference(s) to other database(s): GTC:G12896KI, GlycomeDB:
25489
- Compound ID: 103
|
L-gro-a-D-manHepp-(1-7)-+ a-Kdop-(2-4)-+
| |
a-D-Galp-(1-6)-a-D-Galp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/ |
Show graphically |
Structure type: oligomer
Aglycon: lipid A
Reference(s) to other database(s): GTC:G11346TZ, GlycomeDB:
25490
- Compound ID: 104
|
a-D-Galp-(1-3)-+ a-Kdop-(2-4)-+
| |
L-gro-a-D-manHepp-(1-7)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/ |
Show graphically |
Structure type: oligomer
Aglycon: lipid A
Reference(s) to other database(s): GTC:G38154TL, GlycomeDB:
25491
- Compound ID: 105
|
a-Kdop-(2-4)-+
|
L-gro-a-D-manHepp-(1-7)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/ |
Show graphically |
Structure type: oligomer
Aglycon: lipid A
Trivial name: inner core region of LPS
Compound class: core oligosaccharide
Reference(s) to other database(s): GTC:G07525UL, GlycomeDB:
25492
- Compound ID: 106
Structure type: oligomer
Aglycon: lipid A
Trivial name: outer core region
Compound class: core oligosaccharide, LOS, LPS
Reference(s) to other database(s): GTC:G38337UV, GlycomeDB:
16228
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