The complete structure for the core oligosaccharide region of the water-insoluble low-Mr, lipopolysaccharide of Campylobacter jejuni serotype 0:3 from phenol/water extraction of bacterial cells was assigned through studies on derivatives of the liberated oligosaccharide. Structure determinations were performed using 1H-NMR and 31P-NMR spectroscopies, methylation analysis supported by fast-atom-bombardment mass spectrometry, and Smith degradation experiments. It was concluded that the complete chains in the core oligosaccharide had the following structure in which a proportion of the terminal residues were phosphorylated: . From a similar series of experiments, it was concluded that an associated polysaccharide, which was isolated from the water phase of the phenoVwater extracts, had the following repeating unit in which a proportion of the previously unknown L-glycero-D-ido-heptose (L-a-D-ido-Hep) residues were present as 3-hydroxypropanoyl esters, and were not covalently linked to the lipopolysaccharide: -[ā3)-L-a-D-Ido-Hep-(lā4)-a-D-Gal-(l-]n-.
Lipopolysaccharide, LPS, structure, core, polysaccharide, O-antigen, Campylobacter, Campylobacter jejuni, core region, region
NCBI PubMed ID: 7544281Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Department of Microbiology, University College, Galway, Ireland, Department of Chemistry, York University, Toronto, Ontario, Canada, Carbohydrate Research Centre, Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada
Methods: deacetylation, NMR-2D, FAB-MS, GC-MS, dephosphorylation, GC, Smith degradation
Found