The polysaccharides obtained from the alkali-extractable, water-soluble fraction (F1SS) from the cell wall of Myrothecium verrucaria and Myrothecium atroviride were shown to be composed of β-(1→6)-galactofuranose fully substituted at O-2 by terminal residues of α-glucopyranose and α-glucuronic acid. Glucuronic acid was substituted at O-4 by glucopyranose in the Myrothecium species M. inundatum, M. setiramosum, M. prestonii, M. tongaense and M. roridum. The acidic polysaccharides from Phaeostilbella atra (=Myrothecium atrum) and Myrothecium gramineum lacked the backbone of 2,6 di- O-substituted galactofuranose and contained a high amount of O-5-substituted β-galactofuranose. The structures of the polysaccharides isolated from Myrothecium cincture and Myrothecium penicilloides were unrelated to each other and to the polysaccharides from the other species analysed. The usefulness of these polysaccharides as a characteristic for delimitation of the genus Myrothecium is discussed.
taxonomy, cell-wall polysaccharides, Myrothecium
NCBI PubMed ID: 10816049Publication DOI: 10.1007/s002030000149Journal NLM ID: 0410427Publisher: Berlin, New York: Springer
Correspondence: aleal@cib.csic.es
Institutions: Centro de Investigaciones Biológicas, CSIC, Madrid, Spain, Instituto de Química Orgánica, Departamento de Química Orgánica Biológica, CSIC, Madrid, Spain
Methods: 1H NMR, methylation, GLC-MS, IR, acid hydrolysis, extraction, acetylation, reduction, CC, cell growth, precipitation, phenol-sulfuric acid assay, derivatization, carbazole method
Found