Lipo-oligosaccharide (LOS) from phenol-water extraction of cells of the Campylobacter lari type strain (ATCC35221) was separated as a water-insoluble gel of low relative molecular mass (Mr) from a water-soluble extracellular polysaccharide of high Mr. Structural investigations were performed on the liberated oligosaccharide and the extracellular polysaccharide, variously using 1H, 13C, and 31P NMR spectroscopy, linkage analysis, and fast atom bombardment-mass spectrometry of permethylated derivatives of the glycans and their products of chemical and enzymic degradation. The following structures are proposed for the highly branched region of the LOS: Ac(1-2)bDGlcpN(1-3)[Ac(1-2)]aDGalpN(1-3)aDGalp(1-3)[aDGlcp(1-2)]aXLDmanHepp(1-3)[bDGlcp(1-4),aDGalp(1-2)]aXLDmanHepp(1-5)[xXEtN(1-P-4)]aXKdo(2-5)?XKdo; and for the tetraglycosyl phosphate repeating unit of the extracellular polysaccharide: -P-3)[Ac(1-2)]bDGlcpN(1-2)aL6dgulHepp(1-2)bD3dthrPenp(1-3)aL6dgulHepp(1-.
core, Lipooligosaccharide, LOS, O-antigen, phosphate, Campylobacter, extracellular polysaccharide, ethanolamine, extracellular, 3-deoxy-D-threo-pentose, 6-deoxy-L-gulo-heptose
NCBI PubMed ID: 8593626Publication DOI: 10.1016/0008-6215(95)00284-7Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Carbohydrate Research Centre, Department of Molecular Biology and Medical Genetics, University of Toronto, Toronto, Ontario M5S 1A5, Canada, Department of Chemistry, York University, North York, Toronto, Ontario M3J IP3, Canada
Methods: methylation, NMR-2D, FAB-MS, GLC, Smith degradation
Found