An approach for a rapid identification and characterization of hyaluronic acid (HA) produced by fermentation of Streptococcus equi has been developed. 125-I-labelled hyaluronic acid binding protein (HABP) was used for specific identification of HA in fermentation isolates recognized by HPLC chromatography followed by γ-radiation detection. Simultaneous determination of the molecular weight of HA was carried out also. Further confirmation of HA identification was performed by bovine testes hyaluronidase treatment and HPLC analysis of the products. The analysed sample was identified as almost pure HA with molecular weight approximately 0·8-1·2 × 106 Da, containing protein impurities (5·1%) and some unidentified low molecular weight products. Our approach can be used generally for a rapid identification and characterization of laboratory or industrially-produced HA during its isolation and purification.

• Compound ID: 3236
  

  -3)-b-D-GlcpNAc-(1-4)-b-D-GlcpA-(1-

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  Structure type: suggested polymer biological repeating unit
  Trivial name: hyaluronan, hyaluronic acid, hyaluronan (glycosaminoglycan GAG)
  Compound class: CPS, EPS, glycosaminoglycan, mucopolysaccharide
  Reference(s) to other database(s): GTC:G28158RP, GlycomeDB:899
  
   
  
  Streptococcus equi
  CSDB ID 112947 (all data & tools)