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1. (Article ID: 194)
Vinogradov EV, Pantophlet R, Dijkshoorn L, Brade L, Holst O, Brade H
Structural and serological characterisation of two O-specific polysaccharides of Acinetobacter
European Journal of Biochemistry 239 (1996)
602-610
Extraction of dry bacteria of Acinetobacter strain 34 (DNA group 2) or Acinetobacter strain 108 (DNA group 13) by phenol/water yielded a polymer that was identified by means of serological studies and fatty acid analysis as S-form lipopolysaccharide. Degradation of the lipopolysaccharides of strains 34 and 108 in 1% acetic acid and 5% acetic acid, respectively, and gel-permeation chromatography gave the respective O-antigenic polysaccharides, the structures of which were determined, by compositional analysis and NMR spectroscopy of the polysaccharide, as [Sequence: see text] for strain 108, where D-Fucp3NBuOH represents 3-[(R)-3-hydroxybutyramido] -3,6-dideoxy-D-galactose and D-GalpANAc represents 2-acetamido-2-deoxy-D-galacturonic acid. Both structures were specifically recognised in Western blots by polyclonal rabbit antisera and there was no cross-reaction between these two structures.
Lipopolysaccharide, NMR, Acinetobacter, serology, Western blot
NCBI PubMed ID: 8774703Publication DOI: 10.1111/j.1432-1033.1995.899_3.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Division of Biochemical Microbiology, Center for Medicine and Biosciences, Research Center Borstel, Germany, Department of Medical Microbiology, Leiden University Hospital, Leiden, The Netherlands
Methods: NMR-2D, NMR, composition analysis
The publication contains the following compound(s):
- Compound ID: 728
|
a-L-FucpNAc-(1-4)-+
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-3)-a-D-GalpNAcA-(1-3)-a-L-FucpNAc-(1-3)-b-D-GlcpNAc-(1- |
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Structure type: polymer chemical repeating unit
Compound class: CPS, O-polysaccharide, O-antigen
Reference(s) to other database(s): GTC:G73635WZ, GlycomeDB:
25208
- Compound ID: 730
Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide
Reference(s) to other database(s): GTC:G73528BY, GlycomeDB:
26794
- Compound ID: 729
|
R-3HOBut-(1-3)-b-D-Fucp3N-(1-3)-a-D-GalpNAc-(1-3)-b-D-GalpNAc-(1-3)-+
|
-3)-b-D-GalpNAc-(1-4)-a-D-Galp-(1-6)-b-D-Galp-(1- |
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Structure type: polymer chemical repeating unit
Compound class: CPS, O-polysaccharide, O-antigen
- Compound ID: 731
|
R-3HOBut-(1-3)-b-D-Fucp3N-(1-3)-a-D-GalpNAc-(1-3)-b-D-GalpNAc-(1-3)-+
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-3)-b-D-GalpNAc-(1-4)-a-D-Galp-(1-3)-D-Gro-(1- |
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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide
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