A new water soluble glucan (MGPS), with a molecular weight ~148000 Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography. Its structural characteristics were investigated by acid hydrolysis, methylation analysis, Smith degradation and 1D/2D NMR experiments. The monosaccharide composition analysis showed that MGPS contain only glucose. The backbone of MGPS was composed of two (1→3)-β-D-glucopyranosyl, two (1→6)-β-D-glucopyranosyl, two (1→6)-α-d-glucopyranosyl, and one (1→4)-α-d-glucopyranosyl residues, out of which one (1→3)-β-D-glucopyranosyl residue was branched at O-6 position with terminal α-d-glucopyranosyl residue and one (1→4)-α-d-glucopyranosyl residue branched at O-6 position with terminal β-D-glucopyranosyl residue. In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
glucan, Antioxidant activity, NMR studies, Edible mushrooms, Meripilus giganteus (Pers.) P. Karst
NCBI PubMed ID: 27987828Publication DOI: 10.1016/j.carbpol.2016.11.006Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Islam SS
Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, India, Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, India
Methods: 13C NMR, 1H NMR, periodate oxidation, GLC-MS, acid hydrolysis, GLC, Smith degradation, acetylation, ROESY, TOCSY, methylation analysis, reduction with NaBH4, trifluoroacetic acid solvolysis, DQF-COSY, NOESY, HSQC
Found