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1. (Article ID: 195)
 
Vinogradov EV, Pantophlet R, Haseley SR, Brade L, Holst O, Brade H
Structural and serological characterisation of the O-specific polysaccharide from lipopolysaccharide of Acinetobacter calcoaceticus strain 7 (DNA group 1)
European Journal of Biochemistry 243(1-2) (1997) 167-173
 

S-form lipopolysaccharide was isolated by phenol/water extraction from a strain of Acinetobacter calcoaceticus (DNA group 1). The structure of the O-antigenic polysaccharide was determined by compositional analysis and NMR spectroscopy of the de-O-acylated lipopolysaccharide. The isolated polysaccharide obtained after hydrolysis of lipopolysaccharide in 0.01 M trifluoroacetic acid has the followint structure: [see formula in text] in which Pyr is pyruvate. the O-acetyl substitution of D-Gal was non-stoichiometric. The O-antigen was specifically recognised in wesern blots by polyclonal rabbit antisera.

Lipopolysaccharide, NMR, DNA, Acinetobacter, Acinetobacter calcoaceticus, O-antigen structure, pyruvate

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