A heterogalactan, named ACW0, was extracted from Antrodia camphorata and purified by anion exchange and gel permeation chromatography. It was composed of galactose (94.98%), traces of mannose (2.41%), and fucose (2.61%), with its molecular weight estimated to be 13.5 kDa. The polysaccharide ACW0 was shown to be a mannofucogalactan with a backbone chain of α-d-1,6-linked Gal, attached by a non-reducing terminal α-d-Man and α-l-Fuc on C-2 of nearly every six α-d-1,6-linked Gal residues. A sulfated polysaccharide, ACW0-Sul was achieved by the chlorosulfonic acid-pyridine method. Compared with the native polysaccharide, ACW0-Sul could disrupt tube formation and migration as well as cell growth of human microvascular endothelial cells (HMEC-1) dose-dependently. Further studies revealed that phosphorylation of Extracellular Regulated Protein Kinases (Erk) and Focal Adhesion Kinase (FAK) were significantly inhibited by ACW0-Sul. These results suggested that ACW0-Sul could be a potent candidate for anti-angiogenic agent development.
sulfated polysaccharide, mannofucogalactan, Antrodia camphorata, anti-angiogenesis
Publication DOI: 10.3390/polym9060228Journal NLM ID: 101545357Publisher: Basel: MDPI
Correspondence: Zhu T
; Tian D ; Ding K
Institutions: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China, University of Chinese Academy of Sciences, Beijing, China, Nano Science and Technology Institute, University of Science and Technology of China, Hefei, China, Department of Chemical and Molecular Engineering, Nanjing Tech University, Nanjing, China, State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China
Methods: 13C NMR, 1H NMR, IR, GC-MS, acid hydrolysis, GC, HPLC, acetylation, methylation analysis, reduction with NaBH4, trifluoroacetic acid solvolysis, HPGPC, HMBC, COSY, HSQC
Found